Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/19483
Title: Kinetics of Rispens CV1988 Vaccine Virus in Single and Mixed Infections with very Virulent Marek's Disease Virus in Isa Brown Chickens
Contributor(s): Ralapanawe, Sithara  (author); Walkden-Brown, Steve W  (author)orcid ; Islam, Afm Fakhrul  (author); Renz, Katrin  (author)
Publication Date: 2015
Open Access: Yes
Handle Link: https://hdl.handle.net/1959.11/19483
Open Access Link: http://sydney.edu.au/vetscience/apss/documents/2015/APSS%202015%20Proceedings%20Final.pdfOpen Access Link
Abstract: We report the kinetics of infections with serotype 1 pathogenic Marek's Disease virus (MDV) and Rispens CVI988 (Rispens) vaccine virus in the same host. We conducted a 2x3 factorial experiment with two replicates testing the effects of vaccination with Rispens (vaccinated, unvaccinated) and challenge MDV (isolates MPF57, FT158 or unchallenged) using 236 female ISA Brown chickens. The chickens were vaccinated at hatch and challenged at day 5 of age, and the experimental duration was to 56 days post challenge. Peripheral blood lymphocytes (PBL) and feather samples were collected at regular intervals during the experiment. Extracted DNA was subjected to two quantitative polymerase chain reaction (qPCR) tests specific for either pathogenic MDV1 or Rispens viruses. The results showed that the Rispens vaccination reduced the pathogenic viral load in PBL by 1.4 logs and feathers by 1.2 logs. The viral load of pathogenic MDV was significantly higher in both PBL (1 log) and feather (1.3 logs) than that of Rispens in co infected hosts. The more virulent isolate FT158 had a significantly higher viral load than MPF57 in PBL (1.1 logs), but not in feather.
Publication Type: Conference Publication
Conference Name: 26th Annual Australian Poultry Science Symposium, Sydney, Australia, 9th - 11th February, 2015
Conference Details: 26th Annual Australian Poultry Science Symposium, Sydney, Australia, 9th - 11th February, 2015
Source of Publication: Proceedings of the 26th Annual Australian Poultry Science Symposium, p. 183-186
Publisher: Poultry Research Foundation
Place of Publication: Sydney, Australia
ISSN: 1034-6260
Field of Research (FOR): 070712 Veterinary Virology
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
Other Links: http://sydney.edu.au/vetscience/apss/proceed.shtml
Series Name: Australian Poultry Science Symposium Proceedings
Series Number : 26
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Appears in Collections:Conference Publication
School of Environmental and Rural Science

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