Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/19458
Title: Real-time PCR quantification of infectious laryngotracheitis virus in chicken tissues, faeces, isolator-dust and bedding material over 28 days following infection reveals high levels in faeces and dust
Contributor(s): Roy, Parimal (author); Islam, Afm Fakhrul  (author); Burgess, Susan  (author); Hunt, Peter William  (author); McNally, Jody (author); Walkden-Brown, Steve W  (author)orcid 
Publication Date: 2015
Open Access: Yes
DOI: 10.1099/jgv.0.000268Open Access Link
Handle Link: https://hdl.handle.net/1959.11/19458
Abstract: Infectious laryngotracheitis (ILT) is an important disease of chickens caused by ILT virus (ILTV). We used the Australian SA2 and A20 vaccine strains of ILTV to determine tissue distribution and excretion characteristics of ILTV in specific-pathogen-free chickens and to determine whether ILTV is readily detectable in environmental samples such as faeces, bedding material and dust using real-time quantitative PCR. Three groups of 10 freshly hatched chicks were placed in isolators and infected orally with high doses of the two strains of vaccine virus or left unchallenged as controls. Over a 28-day post-infection (p.i.) period, faecal and serum samples were collected at frequent intervals from six individually identified chickens in each group. Dust and litter samples from the isolators were collected less frequently. Tissue samples were collected from three to four sacrificed or dead/euthanized birds at 6, 14 and 28 days p.i. Infection resulted in clinical ILT, a pronounced antibody response and sustained qPCR detection of the viral genome in the trachea, Harderian gland, lung and kidney up to 28 days p.i. A high level of the viral genome was also detected in faeces between 2 and 7 days p.i., declining by about approximately four orders of magnitude to low, but detectable, levels at 21 and 28 days p.i. The finding of high-level shedding of ILTV in faeces warrants further investigation into the epidemiological role of this, and the sustained high levels of ILTV observed in dust suggest that it may be a useful sample material for monitoring ILTV status in flocks.
Publication Type: Journal Article
Source of Publication: Journal of General Virology, 96(11), p. 3338-3347
Publisher: The Microbiology Society
Place of Publication: United Kingdom
ISSN: 1465-2099
0022-1317
Fields of Research (FoR) 2008: 070708 Veterinary Parasitology
Fields of Research (FoR) 2020: 300909 Veterinary parasitology
Socio-Economic Objective (SEO) 2008: 830309 Poultry
Socio-Economic Objective (SEO) 2020: 100411 Poultry
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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