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https://hdl.handle.net/1959.11/19437
Title: | In vivo characterisation of two Australian isolates of Marek's disease virus including pathology, viral load and neuropathotyping based on clinical signs | Contributor(s): | Wajid, Salih J (author); Walkden-Brown, Steve W (author) ; Vanselow, Barbara (author); Islam, A Fakhrul (author); Renz, Katrin (author) | Publication Date: | 2015 | DOI: | 10.1111/avj.12342 | Handle Link: | https://hdl.handle.net/1959.11/19437 | Abstract: | 'Objective' To evaluate the pathogenicity of Australian Marek's disease virus (MDV) isolate MPF23 (1985) against the reference strain MPF57 based on pathology, viral load and neuropathotyping on the basis of clinical signs. 'Procedure' Two MDV challenge isolates (MPF57 or MPF23) were administered to unvaccinated specific-pathogen free (SPF) layer chicks on day 5 after hatch at three challenge doses (500, 2000 or 8000 plaque-forming units (pfu)/chick). Mortality, body weight, immune organ weights, MDV load in peripheral blood lymphocytes (PBL) andm clinical signs were measured to 56 days post challenge (dpc). 'Results' MPF23 was the more pathogenic of the two viruses, inducing higher mortality (81% vs 62%) and incidence of MD lesions (100% vs 76%). MPF23 induced earlier, more sustained and more severe neurological signs in the period 26-56 dpc. However, there were few differences during the 0-23 dpc used in the neuropathotyping classification under test. The observed pattern during this earlier period classified both viruses as neuropathotype B, consistent with a very virulent pathotype. MDV load in PBL at 7 and 44 dpc did not differ between virus isolates, but the load at 7 dpc was significantly and negatively associated with time to euthanasia or death. 'Conclusion' MPF23 appears to be as, or more, virulent than the MDV strains isolated over the subsequent two decades. The neuropathotyping system developed in the USA did not clearly differentiate between the two isolates under test; however, extension of the period of assessment of clinical signs beyond 26 dpc did reveal clear differences. | Publication Type: | Journal Article | Source of Publication: | Australian Veterinary Journal, 93(7), p. 240-247 | Publisher: | Wiley-Blackwell Publishing Ltd | Place of Publication: | United Kingdom | ISSN: | 1751-0813 0005-0423 |
Fields of Research (FoR) 2008: | 070708 Veterinary Parasitology 070709 Veterinary Pathology |
Fields of Research (FoR) 2020: | 300909 Veterinary parasitology 300910 Veterinary pathology |
Socio-Economic Objective (SEO) 2008: | 830309 Poultry | Socio-Economic Objective (SEO) 2020: | 100411 Poultry | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Environmental and Rural Science |
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