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Title: Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in 'Ziziphus jujuba'
Contributor(s): Li, Lan-Song (author); Meng, Yu-Ping (author); Cao, Qiu-Feng (author); Yang, Yong-Zhen (author); Wang, Fan (author); Jia, Hu-Sheng (author); Wu, Shubiao  (author)orcid ; Liu, Xu-Guang (author)
Publication Date: 2016
Open Access: Yes
DOI: 10.1134/S000629791606002X
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Abstract: Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of 'Ziziphus jujuba'. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in 'Z. jujuba' within 24 h upon Cd exposure. 'Escherichia coli' cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and 'E. coli' cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.
Publication Type: Journal Article
Source of Publication: Biochemistry, 81(6), p. 565-573
Publisher: MAIK Nauka - Interperiodica
Place of Publication: Russian Federation
ISSN: 0006-2979
Field of Research (FOR): 060705 Plant Physiology
Socio-Economic Outcome Codes: 820211 Stone Fruit
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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