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Title: 花青素合成调控转录基因作为直观标记的载体构建及其在玉米幼胚中的瞬时表达
English Title: The Vector Construction of Anthocyanins as a Visual Marker Gene and Its Transient Expression in Maize Immature Embryos
Contributor(s): Zhao, Xingmei (author); Wu, Shubiao  (author)orcid ; Wang, Yixue (author); Cui, Guimei (author); Wang, Xiaoqing (author); Du, Jianzhong (author); Wang, Xiaoli (author); Shang, Yongjin (author); Sun, Yi (author)
Publication Date: 2014
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Abstract: Anthocyanin regulatory factor C1/Bperu genes are constructed respectively with a maize embryo specific promoter GLB1 and a constitutive promoter CaMV35S into plant transformation vectors pGlb1CB and p35SCB, and the recombinant expression vectors were introduced into maize embryos by particle bombardment. Microscopic observation confirmed that the two vectors were actively expressed in maize immature embryo cells. The use of anthocyanins as a marker gene not only could reduce the safety concerns from the public over GMO substantially, but could also be used as a visual indicator for the selection of transgenic plants, which could greatly simplify the screening procedures, improve the efficiency and reduce the costs.
Publication Type: Journal Article
Source of Publication: Shengwu Jishu Tongbao, 2014(4), p. 77-82
Publisher: Zhongguo Nongye Kexueyuan
Place of Publication: Beijing, China
ISSN: 1002-5464
Field of Research (FOR): 060702 Plant Cell and Molecular Biology
070303 Crop and Pasture Biochemistry and Physiology
HERDC Category Description: C2 Non-Refereed Article in a Scholarly Journal
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