Age-related protein and mRNA expression of glutathione peroxidases (GPx) and Hsp-70 in different regions of rat kidney with and without stressor

Title
Age-related protein and mRNA expression of glutathione peroxidases (GPx) and Hsp-70 in different regions of rat kidney with and without stressor
Publication Date
2016
Author(s)
Thiab, Noor Riyadh
King, Nicola
McMillan, Mary
( author )
OrcID: https://orcid.org/0000-0002-2336-3985
Email: mrookle2@une.edu.au
UNE Id une-id:mrookle2
Alghamdi, Othman Ahmed S
Jones, Graham L
( author )
OrcID: https://orcid.org/0000-0002-6435-1542
Email: gjones2@une.edu.au
UNE Id une-id:gjones2
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
American Institute of Mathematical Sciences
Place of publication
United States of America
DOI
10.3934/molsci.2016.2.125
UNE publication id
une:19158
Abstract
Small molecular weight oxygen free radical species (ROS) involved in oxidative stress can cause damage to cellular macromolecules including proteins, DNA and lipids. One of the most important enzymes involved in ROS detoxification is glutathione peroxidase (GPx). Here we study the age-related expression of GPx isoenzymes in various parts of the rat kidney with and without exposure to external oxidative stress. These results are correlated to the age dependent changes in the expression of the chaperone, 'Hsp-70'. Protein and mRNA expression of GPx1 and GPx4 was studied in different regions of the kidney in ageing rats in the presence and absence of the external stressor 0.2 mM H₂O₂. Protein levels were examined by Western blot analysis following detection with appropriate antibodies and mRNA levels were analysed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using appropriate primer sequences. mRNA expression for the chaperone 'Hsp70' was investigated in parallel. After reaching a peak at maturity (12 weeks), GPx1 protein and mRNA levels decreased with age under both control and stress conditions, and were higher in the cortex than in the outer and inner medulla. GPx4 protein and mRNA levels showed few comparable age-related changes. By contrast with the observed age-related decrease in GPx1 expression, chaperone 'Hsp-70' mRNA expression greatly increased with age. These findings suggest that the age-related decline in GPx1 expression in the cortex may be partly offset by a reciprocal change in 'Hsp-70' expression. These results are consistent with the oxidative stress theory of ageing.
Link
Citation
AIMS Molecular Science, 3(2), p. 125-137
ISSN
2372-0301
2372-028X
Start page
125
End page
137

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