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Title: Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep
Contributor(s): Regan, Sheena L P (author); McFarlane, James R  (author)orcid ; O'Shea, Tim (author); Andronicos, Nicholas  (author)orcid ; Arfuso, Frank (author); Dharmarajan, Arun (author); Almahbobi, Ghanim (author)
Publication Date: 2015
Open Access: Yes
DOI: 10.1530/rep-14-0581Open Access Link
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Abstract: The aim of the present studywas to determine the direct cause of the mutation-induced, increased ovulation rate in Booroola Merino (BB) sheep. Granulosa cells were removed from antral follicles before ovulation and post-ovulation from BB (nZ5) and WT (nZ12) Merino ewes. Direct immunofluorescence measurement of mature cell surface receptors using flow cytometry demonstrated a significant up-regulation of FSH receptor (FSHR), transforming growth factor beta type 1, bone morphogenetic protein receptor (BMPR1B), and LH receptor (LHR) in BB sheep. The increased density of FSHR and LHR provide novel evidence of a mechanism for increasing the number of follicles that are recruited during dominant follicle selection. The compounding increase in receptors with increasing follicle size maintained the multiple follicles and reduced the apoptosis, which contributed to a high ovulation rate in BB sheep. In addition, we report a mutation-independent mechanism of down-regulation to reduce receptor density of the leading dominant follicle in sheep. The suppression of receptor density coincides with the cessation of mitogenic growth and steroidogenic differentiation as part of the luteinization of the follicle. The BB mutation-induced attenuation of BMPR1B signaling led to an increased density of the FSHR and LHR and a concurrent reduction in apoptosis to increase the ovulation rate. The role of BMPs in receptor modulation is implicated in the development of multiple ovulations.
Publication Type: Journal Article
Source of Publication: Reproduction, 150(2), p. 151-163
Publisher: BioScientifica Ltd
Place of Publication: United Kingdom
ISSN: 1741-7899
Fields of Research (FoR) 2008: 070206 Animal Reproduction
070201 Animal Breeding
119999 Medical and Health Sciences not elsewhere classified
Fields of Research (FoR) 2020: 300305 Animal reproduction and breeding
300109 Non-genetically modified uses of biotechnology
329999 Other biomedical and clinical sciences not elsewhere classified
Socio-Economic Objective (SEO) 2008: 839999 Animal Production and Animal Primary Products not elsewhere classified
Socio-Economic Objective (SEO) 2020: 109999 Other animal production and animal primary products not elsewhere classified
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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