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Title: Characterisation of the main drivers of intra- and inter- breed variability in the plasma metabolome of dogs
Contributor(s): Lloyd, Amanda J (author); Beckmann, Manfred (author); Tailliart, Kathleen (author); Brown, Wendy  (author)orcid ; Draper, John (author); Allaway, David (author)
Publication Date: 2016
Open Access: Yes
DOI: 10.1007/s11306-016-0997-6Open Access Link
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Abstract: 'Introduction' Dog breeds are a consequence of artificial selection for specific attributes. These closed genetic populations have metabolic and physiological characteristics that may be revealed by metabolomic analysis. 'Objectives' To identify and characterise the drivers of metabolic differences in the fasted plasma metabolome and then determine metabolites differentiating breeds. 'Methods' Fasted plasma samples were collected from dogs maintained under two environmental conditions (controlled and client-owned at home). The former (n = 33) consisted of three breeds (Labrador Retriever, Cocker Spaniel and Miniature Schnauzer) fed a single diet batch, the latter (n = 96), client-owned dogs consisted of 9 breeds (Beagle, Chihuahua, Cocker Spaniel, Dachshund, Golden Retriever, Greyhound, German Shepherd, Labrador Retriever and Maltese) consuming various diets under differing feeding regimens. Triplicate samples were taken from Beagle (n = 10) and Labrador Retriever (n = 9) over 3 months. Non-targeted metabolite fingerprinting wasm performed using flow infusion electrospray-ionization mass spectrometry which was coupled with multivariate data analysis. Metadata factors including age, gender, sexual status, weight, diet and breed were investigated. 'Results' Breed differences were identified in the plasma metabolome of dogs housed in a controlled environment. Triplicate samples from two breeds identified intra-individual variability, yet breed separation was still observed. The main drivers of variance in dogs maintained in the home environment were associated with breed and gender. Furthermore, metabolite signals were identified that discriminated between Labrador Retriever and Cocker Spaniels in both environments. 'Conclusion' Metabolite fingerprinting of plasma samples can be used to investigate breed differences in clientowned dogs, despite added variance of diet, sexual status and environment.
Publication Type: Journal Article
Source of Publication: Metabolomics, 12(4), p. 1-12
Publisher: Springer New York LLC
Place of Publication: United States of America
ISSN: 1573-3890
Fields of Research (FoR) 2008: 070203 Animal Management
070201 Animal Breeding
070204 Animal Nutrition
Fields of Research (FoR) 2020: 300302 Animal management
300305 Animal reproduction and breeding
300303 Animal nutrition
Socio-Economic Objective (SEO) 2008: 839999 Animal Production and Animal Primary Products not elsewhere classified
Socio-Economic Objective (SEO) 2020: 109999 Other animal production and animal primary products not elsewhere classified
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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