Determination of shedding profiles of infectious bursal disease virus in faeces of infected SPF chickens using qRT-PCR and association with levels in host tissues, dust and litter

Title
Determination of shedding profiles of infectious bursal disease virus in faeces of infected SPF chickens using qRT-PCR and association with levels in host tissues, dust and litter
Publication Date
2014
Author(s)
Jayasundara, Kanchana
Walkden-Brown, Steve W
( author )
OrcID: https://orcid.org/0000-0002-0638-5533
Email: swalkden@une.edu.au
UNE Id une-id:swalkden
Katz, Margaret E
Islam, Afm Fakhrul
Renz, Katrin
Burgess, Susan
McNally, Jody
Hunt, Peter
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
World Veterinary Poultry Association (WVPA)
Place of publication
Bangkok, Thailand
UNE publication id
une:18948
Abstract
Australian endemic serotype 1 variant 02/95 and classical 06/95 strains of IBDV were detected in thymus, bursa, caecal tonsils and spleen of maternal a.ntibody free SPF chickens inoculated orally at day 1 of age. High levels of virus from both strains were detected at 7 dpi and lower levels at 14 and 28 dpi. Faecal shedding of both strains was detected from 2 dpi and reached a maximum at 5 dpi followed by a gradual reduction. SPF chickens inoculated with both IBDV strains showed bursal atrophy but atrophy was not significantly different between 2 strains. Sera collected at 7, 13, 21 and 28 dpi indicated active infection. Antibody titre increased from 7 dpi onwards, reaching maximal at 21 dpi. Both IBDV strains were detected in environmental samples: poultry dust and bedding materials. This study reveals that the IBDV 02/95 and 06/95 strains behave similarly in host chickens and that qRT-PCR monitoring of IBDV in environmental samples such as poultry dust or litter is feasible.
Link
Citation
The 2nd WVPA Asian Poultry Health Meeting - Testing & Monitoring: Presented Papers, p. 119-126
Start page
119
End page
126

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