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|Title:||Determination of shedding profiles of infectious bursal disease virus in faeces of infected SPF chickens using qRT-PCR and association with levels in host tissues, dust and litter||Contributor(s):||Jayasundara, Kanchana (author); Walkden-Brown, Steve W (author) ; Katz, Margaret E (author); Islam, Afm Fakhrul (author); Renz, Katrin (author); Burgess, Susan (author); McNally, Jody (author); Hunt, Peter (author)||Publication Date:||2014||Handle Link:||https://hdl.handle.net/1959.11/18746||Abstract:||Australian endemic serotype 1 variant 02/95 and classical 06/95 strains of IBDV were detected in thymus, bursa, caecal tonsils and spleen of maternal a.ntibody free SPF chickens inoculated orally at day 1 of age. High levels of virus from both strains were detected at 7 dpi and lower levels at 14 and 28 dpi. Faecal shedding of both strains was detected from 2 dpi and reached a maximum at 5 dpi followed by a gradual reduction. SPF chickens inoculated with both IBDV strains showed bursal atrophy but atrophy was not significantly different between 2 strains. Sera collected at 7, 13, 21 and 28 dpi indicated active infection. Antibody titre increased from 7 dpi onwards, reaching maximal at 21 dpi. Both IBDV strains were detected in environmental samples: poultry dust and bedding materials. This study reveals that the IBDV 02/95 and 06/95 strains behave similarly in host chickens and that qRT-PCR monitoring of IBDV in environmental samples such as poultry dust or litter is feasible.||Publication Type:||Conference Publication||Conference Name:||2nd WVPA Asia Meeting - Testing & Monitoring, Bangkok, Thailand, 11th - 12th September, 2014||Conference Details:||2nd WVPA Asia Meeting - Testing & Monitoring, Bangkok, Thailand, 11th - 12th September, 2014||Source of Publication:||The 2nd WVPA Asian Poultry Health Meeting - Testing & Monitoring: Presented Papers, p. 119-126||Publisher:||World Veterinary Poultry Association (WVPA)||Place of Publication:||Bangkok, Thailand||Field of Research (FOR):||070712 Veterinary Virology||HERDC Category Description:||E2 Non-Refereed Scholarly Conference Publication||Statistics to Oct 2018:||Visitors: 198
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