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Title: Detection of Novel Biomarkers of Coeliac Disease at the Protein Level using both Fluoro-Immunohistochemistry and Conventional Immunohistochemistry
Contributor(s): Charlesworth, Richard (author); Andronicos, Nicholas  (author)orcid ; Agnew, Linda  (author)orcid 
Publication Date: 2015
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Abstract: Background: Coeliac Disease (CD) is an autoimmune enteropathy targeted against the harmless grain protein gliadin and affects around 1 in 300 people worldwide. The condition causes malabsorptive symptomology and the only current form of treatment is a strict. lifelong gluten-free diet. To date, the exact mechanisms of this autoimmune response have yet to be elucidated. Aims: We have developed and tested a fully-annotated and MIQE compliant 87-gene qRT-PCR array which was specifically designed to investigate the hypothesised immune pathways and processes of active CD. Using duodenal biopsy material from 34 patients with active CD, treated CD and healthy controls; this analysis showed a total of 25 genes which were significantly deferentially expressed between the grades of CD, with a total of 5 genes showing highly significant differential expression and significant linear relationships to the different grades of CD. The aim of this research therefore, was to investigate the expression and localisation of these live genes at the protein level: namely Interferon-y (IFNG), Interleukin 17 (IL17A), Interleukin 18 (IL18), B-lymphocyte Antigen CDI9 (CDI9) and Ectonuelcotide Pyrophosphatasc/Phosphodiesterase 3 (CD203c).
Publication Type: Conference Publication
Conference Name: ASB 2015: 39th Annual Meeting of the Australian Society for Biophysics, Armidale, Australia, 22nd - 25th November, 2015
Conference Details: ASB 2015: 39th Annual Meeting of the Australian Society for Biophysics, Armidale, Australia, 22nd - 25th November, 2015
Source of Publication: 39th Annual Meeting of the Australian Society for Biophysics Delegate Handbook, p. 98-99
Publisher: University of New England
Place of Publication: Armidale, Australia
Field of Research (FOR): 110307 Gastroenterology and Hepatology
110703 Autoimmunity
Socio-Economic Outcome Codes: 920108 Immune System and Allergy
920105 Digestive System Disorders
HERDC Category Description: E3 Extract of Scholarly Conference Publication
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