Detection of Novel Biomarkers of Coeliac Disease at the Protein Level using both Fluoro-Immunohistochemistry and Conventional Immunohistochemistry

Title
Detection of Novel Biomarkers of Coeliac Disease at the Protein Level using both Fluoro-Immunohistochemistry and Conventional Immunohistochemistry
Publication Date
2015
Author(s)
Charlesworth, Richard
Andronicos, Nicholas
( author )
OrcID: https://orcid.org/0000-0001-5881-2296
Email: nandroni@une.edu.au
UNE Id une-id:nandroni
Agnew, Linda
( author )
OrcID: https://orcid.org/0000-0002-2803-0995
Email: lagnew2@une.edu.au
UNE Id une-id:lagnew2
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
University of New England
Place of publication
Armidale, Australia
UNE publication id
une:18664
Abstract
Background: Coeliac Disease (CD) is an autoimmune enteropathy targeted against the harmless grain protein gliadin and affects around 1 in 300 people worldwide. The condition causes malabsorptive symptomology and the only current form of treatment is a strict. lifelong gluten-free diet. To date, the exact mechanisms of this autoimmune response have yet to be elucidated. Aims: We have developed and tested a fully-annotated and MIQE compliant 87-gene qRT-PCR array which was specifically designed to investigate the hypothesised immune pathways and processes of active CD. Using duodenal biopsy material from 34 patients with active CD, treated CD and healthy controls; this analysis showed a total of 25 genes which were significantly deferentially expressed between the grades of CD, with a total of 5 genes showing highly significant differential expression and significant linear relationships to the different grades of CD. The aim of this research therefore, was to investigate the expression and localisation of these live genes at the protein level: namely Interferon-y (IFNG), Interleukin 17 (IL17A), Interleukin 18 (IL18), B-lymphocyte Antigen CDI9 (CDI9) and Ectonuelcotide Pyrophosphatasc/Phosphodiesterase 3 (CD203c).
Link
Citation
39th Annual Meeting of the Australian Society for Biophysics Delegate Handbook, p. 98-99
Start page
98
End page
99

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