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Title: Molecular Tools to Study 'Azospirillum' sp. and Other Related Plant Growth Promoting Rhizobacteria
Contributor(s): Pereg, Lily  (author); McMillan, Mary  (author)orcid 
Publication Date: 2015
DOI: 10.1007/978-3-319-06542-7_4
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Abstract: Molecular methods have been used in the study of 'Azospirillum' and other related PGPRs to carry out gene functional analysis, create gene knockouts, generate genetically engineered strains, and carry out gene expression studies. Genetic transformation has routinely been carried out using conjugation, while chromosomal modifi cations have been performed using unstable, suicide plasmids, or more stable, broad host-range vectors. Gene expression studies are often carried out using promoter-bound reporter genes; however, quantitative methods such as reverse transcribed polymerase chain reaction can now be used to directly study gene expression. In this chapter we describe the common types of vectors used in 'Azospirillum', as well as methods for transformation and mutagenesis. We also describe the use of promoter-bound reporter genes and the applications of quantitative RT-PCR for 'Azospirillum' gene expression studies. Methods for the isolation of DNA and RNA from 'Azospirillum' for use in molecular and gene expression studies are also described.
Publication Type: Book Chapter
Source of Publication: Handbook for Azospirillum: Technical Issues and Protocols, p. 65-82
Publisher: Springer
Place of Publication: Switzerland
ISBN: 9783319065410
Field of Research (FOR): 060503 Microbial Genetics
060504 Microbial Ecology
060501 Bacteriology
Socio-Economic Outcome Codes: 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
970110 Expanding Knowledge in Technology
970106 Expanding Knowledge in the Biological Sciences
HERDC Category Description: B1 Chapter in a Scholarly Book
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Appears in Collections:Book Chapter
School of Science and Technology

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