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Title: Homocysteine Exposure Impairs Myocardial Resistance to Ischaemia Reperfusion and Oxidative Stress
Contributor(s): Almashhadany, Amer (author); Shackebaei, Dareuosh (author); van der Touw, Tom  (author); Jones, Graham L  (author)orcid ; Suleiman, M Saadeh (author); King, Nicola (author)
Publication Date: 2015
Open Access: Yes
DOI: 10.1159/000438582Open Access Link
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Abstract: Background/Aims: Hyperhomocysteinaemia is recognised as a strong independent risk factor for developing cardiovascular disease. This study investigated how an acute homocysteine dose affected cardiac performance during ischaemia reperfusion and cardiomyocyte contractility and morphology under normal conditions and during oxidative stress. Methods: Cardiac function was measured in isolated and perfused rat hearts before and after 40 minutes' global normothermic ischaemia. Where used, 0.1 mM L-homocysteine was present prior to, and throughout ischaemia, before wash out after 10 minutes' reperfusion. Calcium transients under normal conditions and changes in contractile synchronicity during oxidative stress (exposure to 0.2 mM H2O2) were measured in freshly isolated rat cardiomyocytes incubated for 60 minutes ± 0.1 mM L-homocysteine. Results: During ischaemia reperfusion 0.1 mM L-homocysteine significantly reduced the rate pressure product during reperfusion (10,038 ± 749 vs. 5955 ± 567 mmHg bpm, p < 0.001), but did not affect time to ischaemic contracture. Incubation of freshly isolated cardiomyocytes with 0.1 mM L-homocysteine significantly decreased the amplitude of the calcium transient and slowed the time to half relaxation. Conclusions: These findings suggest that homocysteine exposure affected myocardial recovery from ischaemia and contractile homeostasis although the exact mechanisms for these changes remain to be determined.
Publication Type: Journal Article
Source of Publication: Cellular Physiology and Biochemistry, 37(6), p. 2265-2274
Publisher: S Karger AG
Place of Publication: Switzerland
ISSN: 1421-9778
Field of Research (FOR): 060199 Biochemistry and Cell Biology not elsewhere classified
060603 Animal Physiology - Systems
119999 Medical and Health Sciences not elsewhere classified
Socio-Economic Outcome Codes: 920103 Cardiovascular System and Diseases
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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