Effect of ageing on metabolite and oxidant concentrations in different regions of rat kidney under normal and stress conditions

Title
Effect of ageing on metabolite and oxidant concentrations in different regions of rat kidney under normal and stress conditions
Publication Date
2015
Author(s)
Thiab, Noor Riyadh
King, Nicola
Jones, Graham L
( author )
OrcID: https://orcid.org/0000-0002-6435-1542
Email: gjones2@une.edu.au
UNE Id une-id:gjones2
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Springer New York LLC
Place of publication
United States of America
DOI
10.1007/s11010-015-2482-3
UNE publication id
une:18493
Abstract
Metabolic and oxidative stresses have been implicated in ageing and the pathogenesis of chronic kidney disease. In this study, we investigated the glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and lactate concentrations in different kidney regions under control conditions and after exposure to oxidative stress invoked by 0.2 mM H₂O₂. Slices of superficial cortex, outer or inner medulla were dissected from kidneys of male Wistar rats of 5-, 12-, 36- and 60-week old. Samples were incubated for 30 min ± 0.2 mM H2O2 prior to homogenisation and centrifugation. The concentrations of GSH, TBARS and lactate were measured by colorimetry. Each metabolite showed a distinctive pattern. For GSH, this was 12 weeks[ 36 weeks[60 weeks and 5 weeks with the highest concentration measured in the superficial cortex at 12 weeks. For TBARS and lactate, the pattern was for the lowest concentration at 12 weeks and the highest at 60 and 5 weeks. The highest lactate and TBARS concentrations were measured under oxidative stress conditions, particularly at 5 and 60 weeks. These results suggest that GSH in different kidney regions peaks at maturity and then reduces with increasing age.
Link
Citation
Molecular and Cellular Biochemistry, 408(1-2), p. 55-61
ISSN
1573-4919
0300-8177
Start page
55
End page
61

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