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Title: Effect of ageing on metabolite and oxidant concentrations in different regions of rat kidney under normal and stress conditions
Contributor(s): Thiab, Noor Riyadh (author); King, Nicola (author); Jones, Graham L  (author)orcid 
Publication Date: 2015
DOI: 10.1007/s11010-015-2482-3
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Abstract: Metabolic and oxidative stresses have been implicated in ageing and the pathogenesis of chronic kidney disease. In this study, we investigated the glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and lactate concentrations in different kidney regions under control conditions and after exposure to oxidative stress invoked by 0.2 mM H₂O₂. Slices of superficial cortex, outer or inner medulla were dissected from kidneys of male Wistar rats of 5-, 12-, 36- and 60-week old. Samples were incubated for 30 min ± 0.2 mM H2O2 prior to homogenisation and centrifugation. The concentrations of GSH, TBARS and lactate were measured by colorimetry. Each metabolite showed a distinctive pattern. For GSH, this was 12 weeks[ 36 weeks[60 weeks and 5 weeks with the highest concentration measured in the superficial cortex at 12 weeks. For TBARS and lactate, the pattern was for the lowest concentration at 12 weeks and the highest at 60 and 5 weeks. The highest lactate and TBARS concentrations were measured under oxidative stress conditions, particularly at 5 and 60 weeks. These results suggest that GSH in different kidney regions peaks at maturity and then reduces with increasing age.
Publication Type: Journal Article
Source of Publication: Molecular and Cellular Biochemistry, 408(1-2), p. 55-61
Publisher: Springer New York LLC
Place of Publication: New York, United States of America
ISSN: 1573-4919
Field of Research (FOR): 060199 Biochemistry and Cell Biology not elsewhere classified
119999 Medical and Health Sciences not elsewhere classified
060603 Animal Physiology - Systems
Socio-Economic Objective (SEO): 929999 Health not elsewhere classified
920119 Urogenital System and Disorders
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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