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|Title:||Impacts of the Callipyge Mutation on Ovine Plasma Metabolites and Muscle Fibre Type||Contributor(s):||Li, Juan (author); Greenwood, Paul (author); Cockett, Noelle E (author); Hadfield, Tracy S (author); Vuocolo, Tony (author); Byrne, Keren (author); White, Jason D (author); Tellam, Ross L (author); Schirra, Horst Joachim (author)||Publication Date:||2014||Open Access:||Yes||DOI:||10.1371/journal.pone.0099726||Handle Link:||https://hdl.handle.net/1959.11/17809||Abstract:||The ovine Callipyge mutation causes postnatal muscle hypertrophy localized to the pelvic limbs and torso, as well as body leanness. The mechanism underpinning enhanced muscle mass is unclear, as is the systemic impact of the mutation. Using muscle fibre typing immunohistochemistry, we confirmed muscle specific effects and demonstrated that affected muscles had greater prevalence and hypertrophy of type 2X fast twitch glycolytic fibres and decreased representation of types 1, 2C, 2A and/or 2AX fibres. To investigate potential systemic effects of the mutation, proton NMR spectra of plasma taken from lambs at 8 and 12 weeks of age were measured. Multivariate statistical analysis of plasma metabolite profiles demonstrated effects of development and genotype but not gender. Plasma from Callipyge lambs at 12 weeks of age, but not 8 weeks, was characterized by a metabolic profile consistent with contributions from the affected hypertrophic fast twitch glycolytic muscle fibres. Microarray analysis of the perirenal adipose tissue depot did not reveal a transcriptional effect of the mutation in this tissue. We conclude that there is an indirect systemic effect of the Callipyge mutation in skeletal muscle in the form of changes of blood metabolites, which may contribute to secondary phenotypes such as body leanness.||Publication Type:||Journal Article||Source of Publication:||PLoS One, 9(6), p. 1-15||Publisher:||Public Library of Science (PLoS)||Place of Publication:||United States of America||ISSN:||1932-6203||Field of Research (FOR):||070201 Animal Breeding
070204 Animal Nutrition
|Socio-Economic Outcome Codes:||970106 Expanding Knowledge in the Biological Sciences||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Statistics to Oct 2018:||Visitors: 384
|Appears in Collections:||Journal Article|
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