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Title: Identification of a novel microRNA important for melanogenesis in alpaca ('Vicugna pacos')
Contributor(s): Yang, S (author); Fan, R (author); Dong, C (author); Shi, Z (author); Ji, K (author); Zhang, J (author); Wang, H (author); Herrid, Muren  (author); Zhang, Q (author); Yao, J (author); Smith, G W (author)
Publication Date: 2015
Open Access: Yes
DOI: 10.2527/jas.2014-8404Open Access Link
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Abstract: The molecular mechanisms underlying the formation of coat colors in animals are poorly understood. Recent studies have demonstrated that microRNA play important roles in the control of melanogenesis and coat color in mammals. In a previous study, we characterized the miRNA expression profiles in alpaca skin with brown and white coat color and identified a novel miRNA (named lpa-miR-nov-66) that is expressed significantly higher in white skin compared to brown skin. The present study was conducted to determine the functional roles of this novel miRNA in the regulation of melanogenesis in alpaca melanocytes. lpa-miR-nov-66 is predicted to target the soluble guanylate cyclase ('sGC') gene based on presence of a binding site in the sGC coding sequence (CDS). Overexpression of lpa-miR-nov-66 in alpaca melanocyes upregulated the expression of sGC both at the mRNA and protein level. Overexpression of lpa-miR-nov-66 in melanocyes also resulted in decreased expression of key melanogenic genes including tyrosinase ('TYR'), tyrosinase related protein 1 ('TYRP1'), and microphthalmia transcription factor ('MITF'). Our ELISA assays showed increased cyclic guanosine monophosphate (cGMP) but decreased cyclic adenosine monophosphate (cAMP) production in melanocytes overexpressing lpa-miR-nov-66. In addition, overexpression of lpa-miR-nov-66 also reduced melanin production in cultured melanocytes. Results support a role of lpa-miR-nov-66 in melanocytes by directly or indirectly targeting sGC, which regulates melanogenesis via the cAMP pathway.
Publication Type: Journal Article
Source of Publication: Journal of Animal Science, 93(4), p. 1622-1631
Publisher: American Society of Animal Science
Place of Publication: United States of America
ISSN: 0021-8812
Field of Research (FOR): 060407 Genome Structure and Regulation
060602 Animal Physiology - Cell
070201 Animal Breeding
Socio-Economic Objective (SEO): 969999 Environment not elsewhere classified
830307 Minor Livestock (e.g. Alpacas, Ostriches, Crocodiles, Farmed Rabbits)
830505 Raw Wool
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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