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Ussing chambers are frequently used for 'in vitro' evaluation of intestinal transport physiology. The current study describes investigating the jejunal tissue from laying hens using a specific preparation method and evaluates the effect of glutamine in the maintenance buffer. 'Tunica mucosa' was stripped from 104 jejunal samples from 10 hens and stabilised by a net device. Fifty samples were maintained with modified Krebs-Henseleit buffer (Control), 54 samples with additional 5 mM glutamine (Group Gln). The percentage of responding samples varied between 87 and 100%. Mean short circuit current (Δ/sc,) [μA/cm²] of samples exposed to 10 mM glucose in the Control group and Group Gln was 17.0 and 14.6 ('p' = 0.836), respectively, of samples exposed to 100 μM phloridzin -13.3 and -11.8 ('p' = 0.712), respectively, and of samples exposed to 100 μM carbachol 4.7 and 3.7 ('p' = 0.450), respectively. In conclusion, the net-supported method enabled a reliable investigation of jejunum from laying hens. Glutamine in the maintenance buffer was of no significant benefit. |
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