Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/17616
Title: | Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs | Contributor(s): | Ruhnke, Isabelle (author) ; DeBiasio, John V (author); Suchodolski, Jan S (author); Newman, Shelley J (author); Musch, Mark W (author); Steiner, Joerg M (author) | Publication Date: | 2012 | DOI: | 10.1016/j.rvsc.2012.05.009 | Handle Link: | https://hdl.handle.net/1959.11/17616 | Abstract: | Adapter-modified Ussing chambers have been used for assessment of endoscopically obtained intestinal biopsies in humans. The aim of this study was to evaluate the feasibility of an adapter-modified Ussing chamber for assessment of intestinal transport physiology in endoscopically-obtained colonic biopsies from cats and dogs. Fifteen colonic biopsies from four cats and 13 colonic biopsies from four dogs were transferred into a modified Ussing chamber and sequentially exposed to several compounds. Baseline mean ± SD conductance was measured. Changes of short circuit current (Δ/sc) were observed after exposure to glucose (number of feline biopsies that responded = 0/number of canine biopsies that responded = 4), phloridzin ('n' = 0/'n' = 7), histamine ('n' = 5/'n' = 12), serotonin (n = 7/n = 12), prostaglandin (n = 5/n = 7), forskolin (n = 7/n = 7), and ouabain (n = 9/n = 7). The adapter-modified Ussing chamber studied here enables investigation of transport physiology of endoscopically-obtained colonic biopsies from companion animals. However, we observed a large variability of results, suggesting that clinical use of this method is limited. | Publication Type: | Journal Article | Source of Publication: | Research in Veterinary Science, 93(3), p. 1454-1461 | Publisher: | Elsevier Ltd | Place of Publication: | United Kingdom | ISSN: | 1532-2661 0034-5288 |
Fields of Research (FoR) 2008: | 070703 Veterinary Diagnosis and Diagnostics 070706 Veterinary Medicine |
Fields of Research (FoR) 2020: | 300904 Veterinary diagnosis and diagnostics 300907 Veterinary medicine (excl. urology) |
Socio-Economic Objective (SEO) 2008: | 860902 Veterinary Diagnostics | Socio-Economic Objective (SEO) 2020: | 241602 Veterinary diagnostics | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Environmental and Rural Science |
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