Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/17616
Title: Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs
Contributor(s): Ruhnke, Isabelle  (author)orcid ; DeBiasio, John V (author); Suchodolski, Jan S (author); Newman, Shelley J (author); Musch, Mark W (author); Steiner, Joerg M (author)
Publication Date: 2012
DOI: 10.1016/j.rvsc.2012.05.009
Handle Link: https://hdl.handle.net/1959.11/17616
Abstract: Adapter-modified Ussing chambers have been used for assessment of endoscopically obtained intestinal biopsies in humans. The aim of this study was to evaluate the feasibility of an adapter-modified Ussing chamber for assessment of intestinal transport physiology in endoscopically-obtained colonic biopsies from cats and dogs. Fifteen colonic biopsies from four cats and 13 colonic biopsies from four dogs were transferred into a modified Ussing chamber and sequentially exposed to several compounds. Baseline mean ± SD conductance was measured. Changes of short circuit current (Δ/sc) were observed after exposure to glucose (number of feline biopsies that responded = 0/number of canine biopsies that responded = 4), phloridzin ('n' = 0/'n' = 7), histamine ('n' = 5/'n' = 12), serotonin (n = 7/n = 12), prostaglandin (n = 5/n = 7), forskolin (n = 7/n = 7), and ouabain (n = 9/n = 7). The adapter-modified Ussing chamber studied here enables investigation of transport physiology of endoscopically-obtained colonic biopsies from companion animals. However, we observed a large variability of results, suggesting that clinical use of this method is limited.
Publication Type: Journal Article
Source of Publication: Research in Veterinary Science, 93(3), p. 1454-1461
Publisher: Elsevier Ltd
Place of Publication: United Kingdom
ISSN: 1532-2661
0034-5288
Fields of Research (FoR) 2008: 070703 Veterinary Diagnosis and Diagnostics
070706 Veterinary Medicine
Fields of Research (FoR) 2020: 300904 Veterinary diagnosis and diagnostics
300907 Veterinary medicine (excl. urology)
Socio-Economic Objective (SEO) 2008: 860902 Veterinary Diagnostics
Socio-Economic Objective (SEO) 2020: 241602 Veterinary diagnostics
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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