Feed supplemented with organic acids does not affect starch digestibility, nor intestinal absorptive or secretory function in broiler chickens

Ruhnke, Isabelle; Rohe, I; Boroojeni, F Goodarzi; Knorr, F; Mader, A; Hafeez, A; Zentek, J

doi:10.1111/jpn.12313

Author(s)

Ruhnke, Isabelle

Rohe, I

Boroojeni, F Goodarzi

Knorr, F

Mader, A

Hafeez, A

Zentek, J

Publication Date

2015

Abstract

The current study aimed to determine the impact of acidified feed on apparent ileal starch digestibility, intestinal transport and barrier function and intestinal glucose transporter expression. The experiment included a control group and a treatment group with broilers fed a standard diet without or w-35. Starch digestibility was determined using 0.2% titanium dioxide as ingestible marker. Gene expressions of the intestinal sodium glucose transporter 1 (SGLT-1) and glucose transporter 2 (GLUT-2) were analysed using qPCR analysis. Additionally, SGLT-1 function and chloride secretion were analysed in Ussing chamber experiments. Jejunal samples were sequentially exposed to 10 mM glucose, 100 µm phloridzin, 100 µm histamine and 100 µm carbachol. Apparent ileal starch digestibility (±SEM) of the control group (97.5 ± 35%) and the acid-treated group (97.0 ± 59%) did not differ (p = 0.674). The mean tissue conductance of intenstinal samples obtained from the control group and the treatment group was similar [10.6 mS/cm² (±0.68) and 9.4 mS/cm² (±0.80) respectively (p = 0.147)]. The mean short-circuit currents (ΔIsc) of the samples exposed to glucose, phloridzin, histamine and carbachol did not differ (p > 0.05). Additionally, no differences in the expression of SGLT-1 and GLUT-2 could be observed (p = 0.942, p = 0.413). Based on this study, the consumption of feed supplemented with organic acids was not associated with effects on ileal starch digestibility and functional traits of jejunal tissues, indicating that these additives have no major impact on the small intestinal function in broilers.

Citation

Journal of Animal Physiology and Animal Nutrition, 99(Supplement S1), p. 29-35

ISSN

1439-0396

0931-2439

Link

https://hdl.handle.net/1959.11/17604

Language

en

Publisher

Wiley-Blackwell Verlag GmbH

Title

Feed supplemented with organic acids does not affect starch digestibility, nor intestinal absorptive or secretory function in broiler chickens

Type of document

Journal Article

Entity Type

Publication

Author(s)	Ruhnke, Isabelle Rohe, I Boroojeni, F Goodarzi Knorr, F Mader, A Hafeez, A Zentek, J
Publication Date	2015
Abstract	The current study aimed to determine the impact of acidified feed on apparent ileal starch digestibility, intestinal transport and barrier function and intestinal glucose transporter expression. The experiment included a control group and a treatment group with broilers fed a standard diet without or w-35. Starch digestibility was determined using 0.2% titanium dioxide as ingestible marker. Gene expressions of the intestinal sodium glucose transporter 1 (SGLT-1) and glucose transporter 2 (GLUT-2) were analysed using qPCR analysis. Additionally, SGLT-1 function and chloride secretion were analysed in Ussing chamber experiments. Jejunal samples were sequentially exposed to 10 mM glucose, 100 µm phloridzin, 100 µm histamine and 100 µm carbachol. Apparent ileal starch digestibility (±SEM) of the control group (97.5 ± 35%) and the acid-treated group (97.0 ± 59%) did not differ (p = 0.674). The mean tissue conductance of intenstinal samples obtained from the control group and the treatment group was similar [10.6 mS/cm² (±0.68) and 9.4 mS/cm² (±0.80) respectively (p = 0.147)]. The mean short-circuit currents (ΔIsc) of the samples exposed to glucose, phloridzin, histamine and carbachol did not differ (p > 0.05). Additionally, no differences in the expression of SGLT-1 and GLUT-2 could be observed (p = 0.942, p = 0.413). Based on this study, the consumption of feed supplemented with organic acids was not associated with effects on ileal starch digestibility and functional traits of jejunal tissues, indicating that these additives have no major impact on the small intestinal function in broilers.
Citation	Journal of Animal Physiology and Animal Nutrition, 99(Supplement S1), p. 29-35
ISSN	1439-0396 0931-2439
Link	https://hdl.handle.net/1959.11/17604
Language	en
Publisher	Wiley-Blackwell Verlag GmbH
Title	Feed supplemented with organic acids does not affect starch digestibility, nor intestinal absorptive or secretory function in broiler chickens
Type of document	Journal Article
Entity Type	Publication

Feed supplemented with organic acids does not affect starch digestibility, nor intestinal absorptive or secretory function in broiler chickens

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