Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/17282
Title: Distribution and genetic diversity of 'Tomato spotted wilt virus' following an incursion into Kenya
Contributor(s): Macharia, Isaac (author); Backhouse, David  (author)orcid ; Ateka, Elijah Miinda (author); Wu, Shubiao  (author)orcid ; Harvey, Jagger (author); Njahira, Moses (author); Skilton, Robert A (author)
Publication Date: 2015
DOI: 10.1111/aab.12201
Handle Link: https://hdl.handle.net/1959.11/17282
Abstract: 'Tomato spotted wilt virus' (TSWV) affects the production of many horticultural crops worldwide. It was first reported from Kenya in 1999. The occurrence, distribution and genetic diversity of TSWV were evaluated in four tomato production areas in Kenya a decade after this incursion. The awareness of TSWV and its vectors among farmers was assessed through a questionnaire while plant samples including tomato leaves and fruit were collected from diseased and non-diseased plants. The samples were assayed for TSWV using ELISA and reverse transcriptase-polymerase chain reaction, and the resulting positive samples were sequenced. There was less awareness of the virus, its vectors and alternate hosts among farmers, despite the occurrence of the disease for over a decade. A total of 89 of 408 tomato samples tested positive for TSWV. Most of the positive samples came from Nakuru, where the virus was first detected, with only a single positive sample being obtained from Loitokitok and none from Bungoma or Kirinyaga. A phylogenetic analysis based on partial nucleocapsid (N) protein gene sequences suggested that the Kenyan isolates formed a single subgroup nested within a cluster of isolates that came predominantly from Europe. This indicated a single introduction that had undergone limited diversification. The study revealed that the disease has persisted in the area to which it was first introduced but has had very limited dispersal to other areas.
Publication Type: Journal Article
Source of Publication: Annals of Applied Biology, 166(3), p. 520-529
Publisher: Wiley-Blackwell Publishing Ltd
Place of Publication: United Kingdom
ISSN: 1744-7348
0003-4746
Fields of Research (FoR) 2008: 060704 Plant Pathology
070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
070603 Horticultural Crop Protection (Pests, Diseases and Weeds)
Fields of Research (FoR) 2020: 310805 Plant pathology
300409 Crop and pasture protection (incl. pests, diseases and weeds)
300804 Horticultural crop protection (incl. pests, diseases and weeds)
Socio-Economic Objective (SEO) 2008: 820215 Vegetables
Socio-Economic Objective (SEO) 2020: 260512 Protected vegetable crops
260505 Field grown vegetable crops
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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