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https://hdl.handle.net/1959.11/16522
Title: | Identification of Salt-Stress-Induced Genes from the RNA-Seq Data of 'Reaumuria trigyna' Using Differential Disply Reverse Transcription PCR | Contributor(s): | Dang, Zhen-hua (author); Qi, Qi (author); Zhang, Hui-rong (author); Li, Hao-yu (author); Wu, Shu-Biao (author) ; Wang, Ying-chun (author) | Publication Date: | 2014 | Open Access: | Yes | DOI: | 10.1155/2014/381501 | Handle Link: | https://hdl.handle.net/1959.11/16522 | Abstract: | Next generation sequencing (NGS) technologies have been used to generate huge amounts of sequencing data from many organisms. However, the correct choice of candidate genes and prevention of false-positive results computed from digital gene expression (DGE) of RNA-seq data are vital when using these genetic resources. We indirectly identified 18 salt-stress-induced 'Reaumuria trigyna' transcripts from the transcriptome sequencing data using differential-display reverse transcription PCR (DDRT-PCR) combined with local BLAST searches. Highly consistent with the DGE results, the quantitative real-time PCR expression patterns of these transcripts showed strong upregulation by salt stress, suggesting that these genes may play important roles in 'R. trigyna''s survival under high-salt environments. The method presented here successfully identified responsive genes from the massive amount of RNA-seq data. Thus, we suggest that DDRT-PCR could be employed to mine NGS data in a wide range of applications in transcriptomic studies. In addition, the genes identified in the present study are promising candidates for further elucidation of the salt tolerance mechanisms in 'R. trigyna'. | Publication Type: | Journal Article | Source of Publication: | International Journal of Genomics, v.2014, p. 1-7 | Publisher: | Hindawi Publishing Corporation | Place of Publication: | United States of America | ISSN: | 2314-4378 2314-436X |
Fields of Research (FoR) 2008: | 060705 Plant Physiology | Fields of Research (FoR) 2020: | 310806 Plant physiology | Socio-Economic Objective (SEO) 2008: | 961309 Remnant Vegetation and Protected Conservation Areas in Sparseland, Permanent Grassland and Arid Zone Environments | Socio-Economic Objective (SEO) 2020: | 180604 Rehabilitation or conservation of terrestrial environments | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Environmental and Rural Science |
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