Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/16481
Title: Effects of α-lipoic Acid on mtDNA Damage after Isolated Muscle Contractions
Contributor(s): Fogarty, Mark C (author); de Vito, Giuseppe  (author); Hughes, Ciara M (author); Burke, George (author); Brown, John C (author); McEneny, Jane (author); Brown, David (author); McClean, Conor (author); Davison, Gareth W (author)
Publication Date: 2013
DOI: 10.1249/MSS.0b013e31828bf31e
Handle Link: https://hdl.handle.net/1959.11/16481
Abstract: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, it is not known if α-lipoic acid supplementation can protect against DNA damage after high-intensity isolated quadriceps exercise. This randomized controlled investigation was designed to test the hypothesis that 14 d of α-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n = 12) apparently healthy male participants (age = 28 ± 10 yr, stature = 177 ± 12 cm and body mass = 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000mg of α-lipoic acid (2 x 500-mg tablets) for 14 d (n = 6) or receive no supplement (n = 6) in a double-blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and after the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°.s⁻¹). Results: Exercise increased mitochondrial 8-hydroxy-2-deoxyguanosine (8-OHdG) concentration in both groups (P G 0.05 vs rest) with a concomitant decrease in total antioxidant capacity (P G 0.05 vs rest). There was a marked increase in blood total antioxidant capacity after oral α-lipoic acid supplementation (P < 0.05 vs nonsupplemented), whereas DNA damage (Comet assay and 8-OHdG), lipid peroxidation, and hydrogen peroxide increased after exercise in the non-supplemented group only (P < 0.05 vs supplemented). Exercise increased protein oxidation in both groups (P < 0.05 vs rest). Conclusions: These findings suggest that short-term α-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.
Publication Type: Journal Article
Source of Publication: Medicine and Science in Sports and Exercise, 45(8), p. 1469-1477
Publisher: Lippincott Williams & Wilkins
Place of Publication: United States of America
ISSN: 1530-0315
0195-9131
Fields of Research (FoR) 2008: 110604 Sports Medicine
110602 Exercise Physiology
Fields of Research (FoR) 2020: 320225 Sports medicine
420702 Exercise physiology
Socio-Economic Objective (SEO) 2008: 970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
Socio-Economic Objective (SEO) 2020: 280102 Expanding knowledge in the biological sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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