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Title: Cellular Responses during Morphological Transformation in 'Azospirillum brasilense' and Its flcA Knockout Mutant
Contributor(s): Hou, Xingsheng (author); McMillan, Mary  (author)orcid ; Coumans-Moens, Joelle  (author)orcid ; Poljak, Anne (author); Raftery, Mark (author); Pereg, Lily  (author)
Publication Date: 2014
Open Access: Yes
DOI: 10.1371/journal.pone.0114435Open Access Link
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Abstract: FlcA is a response regulator controlling flocculation and the morphological transformation of 'Azospirillum' cells from vegetative to cyst-like forms. To understand the cellular responses of 'Azospirillum' to conditions that cause morphological transformation, proteins differentially expressed under flocculation conditions in 'A. brasilense' Sp7 and its flcA knockout mutant were investigated. Comparison of 2-DE protein profiles of wild-type (Sp7) and a flcA deletion mutant (Sp7-flcAΔ) revealed a total of 33 differentially expressed 2-DE gel spots, with 22 of these spots confidently separated to allow protein identification. Analysis of these spots by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MASCOT database searching identified 48 proteins (≥10% emPAI in each spot). The functional characteristics of these proteins included carbon metabolism (beta-ketothiolase and citrate synthase), nitrogen metabolism (Glutamine synthetase and nitric oxide synthase), stress tolerance (superoxide dismutase, Alkyl hydroperoxidase and ATP-dependent Clp protease proteolytic subunit) and morphological transformation (transducer coupling protein). The observed differences between Sp7 wild-type and flcA⁻ strains enhance our understanding of the morphological transformation process and help to explain previous phenotypical observations. This work is a step forward in connecting the Azospirillum phenome and genome.
Publication Type: Journal Article
Source of Publication: PLoS One, 9(12), p. 1-25
Publisher: Public Library of Science (PLoS)
Place of Publication: United States of America
ISSN: 1932-6203
Field of Research (FOR): 060504 Microbial Ecology
060501 Bacteriology
060503 Microbial Genetics
Socio-Economic Outcome Codes: 970106 Expanding Knowledge in the Biological Sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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