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Hydrodistilled essential oils and dichloromethane (DCM) extracted volatiles were taken from cultivated specimens of 'Prostanthera centralis', endemic to central Australia. All volatiles were chemically characterised by Gas Chromatography-Mass Spectroscopy (GC-MS) with the use of authentic standards, followed by Nuclear Magnetic Resonance (NMR) Spectroscopy. The antimicrobial activity of the essential oils was measured against a range of Gram-negative and Gram-positive bacterial species using a micro-titre plate broth dilution assay. Twenty-two compounds were identified as components of the sweet smelling aromatic essential oil and DCM extracts, both showing a relatively high abundance of prostantherol. The volatiles extracted using DCM, differed only in the relative abundance of the major components and the lack of ledol and squamulosone. This study constitutes the first time ledol and squamulosone have been identified in a 'Prostanthera' species. Antimicrobial assays showed moderate to high inhibitory activity against some Gram-positive bacteria and the yeast 'Candida albicans'. |
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