Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/15985
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dc.contributor.authorNolan, John Ven
dc.contributor.authorLeng, Ronalden
dc.contributor.authorDobos, Robin Cen
dc.contributor.authorBoston, Raymonden
dc.date.accessioned2014-10-30T13:23:00Z-
dc.date.issued2014-
dc.identifier.citationAnimal Production Science, 54(11-12), p. 2082-2088en
dc.identifier.issn1836-5787en
dc.identifier.issn1836-0939en
dc.identifier.urihttps://hdl.handle.net/1959.11/15985-
dc.description.abstractA procedure is described for solving an open, fully exchanging, three-compartment model representing ruminal volatile fatty acids (VFA) kinetics in sheep. This model was solved using results from a published study in which labelled VFA, viz. 1-¹⁴C-acetate (Ac), 1-¹⁴C-propionate (Pr) and 1-¹⁴C-butyrate (Bu), were infused individually at a constant rate for 240 min into the rumen of sheep on different occasions. During the tracer infusions, the sheep were given 75 g of lucerne ('Medicago sativa') every hour. The patterns of increasing specific radioactivity (SA) during the infusions were described by differential equations that were solved using the computer software, WinSAAM. This linear kinetic analysis gave estimates of the rates of synthesis and absorption of Ac, Pr and Bu and the carbon interconversions between each acid. The sizes of the Ac, Pr and Bu compartments (10.5, 3.9 and 2.1 g C respectively), were also estimated, which is not possible with commonly used algebraic analyses. The model output showed that tracer : tracee equilibrium (plateau SA) had not been reached in the Ac, Pr and Bu compartments during the 240 min of tracer infusion and the algebraic method of analysis used in the original study was therefore compromised. The procedures described here eliminated this source of error; in addition, confidence in the model solution was improved because all data representing the build-up to plateauSAwere used, rather than just estimates of 'plateau' SA. After accounting for VFA interconversions, the rates of VFA absorption (or incorporation into other materials such as microbial polymers or methane) were 85, 48 and 49 g C/day, or 78%,91%and73% of the total production of each VFA, respectively. The variability in the observed SA responses to the tracer infusions was relatively large and this is discussed. Model solutions using the linear kinetic analysis and the previously widely used algebraic analyses are compared. The effect of positional labelling in tracer VFA is also discussed in the light of evidence that the rate of absorption of Pr may be overestimated when [1-¹⁴C]-Pr rather than [2-¹⁴C]-Pr or uniformly labelled [¹⁴C]-Pr is used as the Pr tracer.en
dc.languageenen
dc.publisherCSIRO Publishingen
dc.relation.ispartofAnimal Production Scienceen
dc.titleThe production of acetate, propionate and butyrate in the rumen of sheep: fitting models to ¹⁴C- or ¹³C-labelled tracer data to determine synthesis rates and interconversionsen
dc.typeJournal Articleen
dc.identifier.doi10.1071/AN14539en
dc.subject.keywordsAnimal Nutritionen
local.contributor.firstnameJohn Ven
local.contributor.firstnameRonalden
local.contributor.firstnameRobin Cen
local.contributor.firstnameRaymonden
local.subject.for2008070204 Animal Nutritionen
local.subject.seo2008839999 Animal Production and Animal Primary Products not elsewhere classifieden
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolAnimal Scienceen
local.profile.emailjnolan@une.edu.auen
local.profile.emailrleng3@une.edu.auen
local.profile.emailrdobos2@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20141003-174023en
local.publisher.placeAustraliaen
local.format.startpage2082en
local.format.endpage2088en
local.identifier.scopusid84908430478en
local.peerreviewedYesen
local.identifier.volume54en
local.identifier.issue11-12en
local.title.subtitlefitting models to ¹⁴C- or ¹³C-labelled tracer data to determine synthesis rates and interconversionsen
local.contributor.lastnameNolanen
local.contributor.lastnameLengen
local.contributor.lastnameDobosen
local.contributor.lastnameBostonen
dc.identifier.staffune-id:jnolanen
dc.identifier.staffune-id:rleng3en
dc.identifier.staffune-id:rdobos2en
local.profile.orcid0000-0001-7949-950Xen
local.profile.orcid0000-0002-9110-6729en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:16222en
local.identifier.handlehttps://hdl.handle.net/1959.11/15985en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleThe production of acetate, propionate and butyrate in the rumen of sheepen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorNolan, John Ven
local.search.authorLeng, Ronalden
local.search.authorDobos, Robin Cen
local.search.authorBoston, Raymonden
local.uneassociationUnknownen
local.year.published2014en
local.subject.for2020300303 Animal nutritionen
local.subject.seo2020109999 Other animal production and animal primary products not elsewhere classifieden
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