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Title: Claudin-8 expression in Sertoli cells and putative spermatogonial stem cells in the bovine testis
Contributor(s): McMillan, Mary  (author)orcid ; Andronicos, Nicholas  (author)orcid ; Davey, Rhonda  (author); Stockwell, Sally (author); Hinch, Geoffrey  (author)orcid ; Schmoelzl, Sabine  (author)
Publication Date: 2014
DOI: 10.1071/RD12259
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Abstract: Adhesion molecules are expressed by both adult and embryonic stem cells, with different classes of adhesion molecules involved in cell-membrane and intercellular contacts. In this study the expression of the adhesion molecule claudin-8 (CLDN8), a tight-junction protein, was investigated as a potential marker for undifferentiated spermatogonia in the bovine testis. We found that CLDN8 was expressed by both spermatogonia and a subset of Sertoli cells in the bovine testis. We also showed co-expression of GFRα1 in testis cells with CLDN8 and with 'Dolichos biflorus' agglutinin-fluorescein isothiocyanate (DBA-FITC) staining. We observed co-enrichment of spermatogonia and CLDN8-expressing Sertoli cells in DBA-FITC-assisted magnetic-activated cell sorting (MACS), an observation supported by results from fluorescence-activated cell sorting analysis, which showed CLDN8-expressing cells were over-represented in the MACS-positive cell fraction, leading to the hypothesis that CLDN8 may play a role in the spermatogonial stem-cell niche.
Publication Type: Journal Article
Source of Publication: Reproduction, Fertility and Development, 26(5), p. 633-644
Publisher: CSIRO Publishing
Place of Publication: Collingwood, Australia
ISSN: 1448-5990
Field of Research (FOR): 060802 Animal Cell and Molecular Biology
060602 Animal Physiology - Cell
060106 Cellular Interactions (incl Adhesion, Matrix, Cell Wall)
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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