Please use this identifier to cite or link to this item:
Title: The histomorphometric changes produced by 'Eimeria' and fishmeal in a broiler necrotic enteritis challenge model
Contributor(s): Wu, Shubiao (author)orcid ; Cui, GM (author); Rodgers, Nicholas (author); Sun, Y (author); Swick, Robert A (author)orcid 
Publication Date: 2014
Handle Link:
Abstract: Factors predisposing necrotic enteritis in broiler chickens are complex and their respective influences on the morphological changes of gut epithelial layer have not been widely investigated. A 2 x 2 x 2 factorial experiment with or without dietary fishmeal, 'Eimeria' inoculation and 'Clostridium perfringens' challenges was performed with each treatment having six replicates and each replicate containing 25 birds to investigate the influence of these factors on gut morphology. Challenge of the birds by 'C. perfringens' was carried out on d 14 and 15. Duodenal histomorphometrics were conducted using standard paraffin sectioning procedure and the villus height and crypt depth were measured using optical microscopy. The results showed no effects of dietary fishmeal inclusion or 'Eimeria' inoculation or their interaction prior to the 'C. perfringens' challenge, i.e., at d 13. At d 16, 'Eimeria' inoculation significantly reduced villus height (P < 0.001), while 250 g/kg dietary fishmeal significantly increased the crypt depth (P < 0.05). Furthermore, 'Eimeria' inoculation significantly reduced villus height to crypt depth ratio. 'C. perfringens' challenge on d 14 and 15 did not alter the morphometric characteristics. The morphometric data presented herein confirms previous suggestions that 'Eimeria' inoculation is more important than dietary fishmeal inclusion in predisposing birds to necrotic enteritis under experimental conditions.
Publication Type: Conference Publication
Conference Name: 25th Annual Australian Poultry Science Symposium, Sydney, Australia, 16th - 19th February, 2014
Conference Details: 25th Annual Australian Poultry Science Symposium, Sydney, Australia, 16th - 19th February, 2014
Source of Publication: Proceedings of the 25th Annual Australian Poultry Science Symposium, p. 85-88
Publisher: Poultry Research Foundation, University of Sydney
Place of Publication: Sydney, Australia
ISSN: 1034-6260
Field of Research (FOR): 070204 Animal Nutrition
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
Other Links:
Series Name: Australian Poultry Science Symposium Proceedings
Series Number : 25
Statistics to Oct 2018: Visitors: 155
Views: 158
Downloads: 1
Appears in Collections:Conference Publication

Files in This Item:
3 files
File Description SizeFormat 
Show full item record

Page view(s)

checked on Apr 23, 2019
Google Media

Google ScholarTM






Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.