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Title: A new use of the biosensor Di-C₃-(3) to measure dipeptide transport across the apical membrane of the renal proximal tubule
Contributor(s): Alghamdi, Othman Ahmed S (author); Moens, Pierre  (author)orcid ; Jones, Graham L  (author)orcid ; King, Nicola  (author)
Publication Date: 2013
DOI: 10.1007/s00726-013-1540-y
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Abstract: PEPT1 and PEPT2 are H+-dependent cotransporters found in the kidney. They are important for the reabsorption of small peptides and are also capable of transporting a broad range of peptidomimetic drugs. Due to the electrogenic nature of these carriers, transport of individual substrates should be accompanied by a positive change in the membrane potential difference (pd). The aim of this study was to investigate a novel use for the potential sensitive fluorescent dye 3,30-dipropylthiacarbocyanine iodide [DiS-C₃-(3)] as a biosensor for dipeptide transport across the renal proximal tubule apical membrane. Brush border (BBMV) and outer medulla (OMMV) membrane vesicles were isolated from male Wistar rats using standard techniques. Fluorescence changes were measured when extracellular media at pH 6.6 containing 0-3 mM L-glycyl-L-glutamine (gly-gln) was added to a cuvette containing vesicles pre-equilibrated at pH 7.4 and 7.5 μM DiS-C₃-(3). Data presented are means ± SE of n = 6. A positive change in fluorescence occurred upon gly-gln addition reflecting the expected positive change in membrane pd.
Publication Type: Conference Publication
Conference Name: 13th International Congress on Amino Acids, Peptides and Proteins, Galveston, United States of America, 5th - 7th October, 2013
Conference Details: 13th International Congress on Amino Acids, Peptides and Proteins, Galveston, United States of America, 5th - 7th October, 2013
Source of Publication: Amino Acids, 45(3), p. 588-588
Publisher: Springer Wien
Place of Publication: Austria
ISSN: 1438-2199
Field of Research (FOR): 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Category Description: E3 Extract of Scholarly Conference Publication
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