A new use of the biosensor Di-C₃-(3) to measure dipeptide transport across the apical membrane of the renal proximal tubule

Title
A new use of the biosensor Di-C₃-(3) to measure dipeptide transport across the apical membrane of the renal proximal tubule
Publication Date
2013
Author(s)
Alghamdi, Othman Ahmed S
Moens, Pierre
( author )
OrcID: https://orcid.org/0000-0003-3121-5306
Email: pmoens@une.edu.au
UNE Id une-id:pmoens
Jones, Graham L
( author )
OrcID: https://orcid.org/0000-0002-6435-1542
Email: gjones2@une.edu.au
UNE Id une-id:gjones2
King, Nicola
Editor
Editor(s): Guoyao Wu
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
Springer Wien
Place of publication
Austria
UNE publication id
une:15307
Abstract
PEPT1 and PEPT2 are H+-dependent cotransporters found in the kidney. They are important for the reabsorption of small peptides and are also capable of transporting a broad range of peptidomimetic drugs. Due to the electrogenic nature of these carriers, transport of individual substrates should be accompanied by a positive change in the membrane potential difference (pd). The aim of this study was to investigate a novel use for the potential sensitive fluorescent dye 3,30-dipropylthiacarbocyanine iodide [DiS-C₃-(3)] as a biosensor for dipeptide transport across the renal proximal tubule apical membrane. Brush border (BBMV) and outer medulla (OMMV) membrane vesicles were isolated from male Wistar rats using standard techniques. Fluorescence changes were measured when extracellular media at pH 6.6 containing 0-3 mM L-glycyl-L-glutamine (gly-gln) was added to a cuvette containing vesicles pre-equilibrated at pH 7.4 and 7.5 μM DiS-C₃-(3). Data presented are means ± SE of n = 6. A positive change in fluorescence occurred upon gly-gln addition reflecting the expected positive change in membrane pd.
Link
Citation
Amino Acids, 45(3), p. 588-588
ISSN
1438-2199
0939-4451
Start page
588
End page
588

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