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Title: Effect of ageing and oxidative stress on the glutathione concentration in different kidney regions
Contributor(s): Thiab, Noor Riyadh (author); Jones, Graham L  (author)orcid ; King, Nicola  (author)
Publication Date: 2013
DOI: 10.1007/s00726-013-1540-y
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Abstract: Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. Glutathione (GSH) is the major intracellular thiol in the kidney and an indicator of this organ's redox status. The aim of this study was to investigate how the GSH concentration is affected by age and oxidative stress in different kidney regions. Kidneys were dissected from Male Wistar rats of 5, 12, 36 and 60 weeks-old. Slices of superficial cortex, outer or inner medulla were incubated for 30 min ± 0.2 mM H₂O₂ prior to homogenisation and centrifugation. GSH concentrations were measured colormetrically using a kit. Data are presented in nmol/mg protein, are means ± SE of n = 5 and statistical comparisons were carried out using ANOVA with an appropriate post-test. In all regions and all conditions the GSH concentration showed a similar pattern with 12 weeks-old > 36 weeks-old > 60 weeks-old and 5 weeks-old. The greatest concentration was measured in the 12 weeks-old with the superficial cortex (1509.16 ± 27.34) significantly greater than outer (851.67 ± 47.39, p
Publication Type: Conference Publication
Conference Name: 13th International Congress on Amino Acids, Peptides and Proteins, Galveston, United States of America, 5th - 7th October, 2013
Conference Details: 13th International Congress on Amino Acids, Peptides and Proteins, Galveston, United States of America, 5th - 7th October, 2013
Source of Publication: Amino Acids, 45(3), p. 587-588
Publisher: Springer Wien
Place of Publication: Austria
ISSN: 0939-4451
Field of Research (FOR): 060199 Biochemistry and Cell Biology not elsewhere classified
Socio-Economic Outcome Codes: 920119 Urogenital System and Disorders
HERDC Category Description: E3 Extract of Scholarly Conference Publication
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