Effect of ageing and oxidative stress on the glutathione concentration in different kidney regions

Title
Effect of ageing and oxidative stress on the glutathione concentration in different kidney regions
Publication Date
2013
Author(s)
Thiab, Noor Riyadh
Jones, Graham L
( author )
OrcID: https://orcid.org/0000-0002-6435-1542
Email: gjones2@une.edu.au
UNE Id une-id:gjones2
King, Nicola
Editor
Editor(s): Guoyao Wu
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
Springer Wien
Place of publication
Austria
UNE publication id
une:15306
Abstract
Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. Glutathione (GSH) is the major intracellular thiol in the kidney and an indicator of this organ's redox status. The aim of this study was to investigate how the GSH concentration is affected by age and oxidative stress in different kidney regions. Kidneys were dissected from Male Wistar rats of 5, 12, 36 and 60 weeks-old. Slices of superficial cortex, outer or inner medulla were incubated for 30 min ± 0.2 mM H₂O₂ prior to homogenisation and centrifugation. GSH concentrations were measured colormetrically using a kit. Data are presented in nmol/mg protein, are means ± SE of n = 5 and statistical comparisons were carried out using ANOVA with an appropriate post-test. In all regions and all conditions the GSH concentration showed a similar pattern with 12 weeks-old > 36 weeks-old > 60 weeks-old and 5 weeks-old. The greatest concentration was measured in the 12 weeks-old with the superficial cortex (1509.16 ± 27.34) significantly greater than outer (851.67 ± 47.39, p
Link
Citation
Amino Acids, 45(3), p. 587-588
ISSN
1438-2199
0939-4451
Start page
587
End page
588

Files:

NameSizeformatDescriptionLink