Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/14258
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dc.contributor.authorChen, Yen
dc.contributor.authorArthur, Paul Fen
dc.contributor.authorHerd, Robert Men
dc.contributor.authorQuinn, Kimen
dc.contributor.authorBarchia, I Men
dc.date.accessioned2014-03-13T14:20:00Z
dc.date.issued2012en
dc.identifier.citationAnimal Production Science, 52(7), p. 608-612en
dc.identifier.issn1836-5787en
dc.identifier.issn1836-0939en
dc.identifier.urihttps://hdl.handle.net/1959.11/14258en
dc.description.abstractFeed efficiency is an economically important trait in livestock and residual feed intake (RFI) is a commonly used measure of the trait in beef cattle. Residual feed intake is the difference between the actual feed intake recorded over a test period and the expected feed intake of an animal based on its size and growth rate. It is a heritable trait, and efficient animals have lower RFI values. Several genes have been identified as being differentially expressed in the liver of Angus bulls that have been divergently selected for RFI. The objective of this study was to use genes that are differentially expressed in bulls to classify Angus steers from the same divergent RFI selection lines. Liver samples were collected at slaughter from 40 high RFI and 40 low RFI steers that were ≈23 months old, and had just completed a 251-day feedlot feeding period. RNA samples from the livers were assayed by quantitative real-time PCR for 14 genes, which have been identified previously in bulls. Steers were not measured for RFI, hence the estimated breeding values (EBV) for RFI of their parents were used to calculate their mid-parent (average of the two parents) RFI-EBV. Correlation and discriminant analyses were conducted on the normalised quantitative real-time PCR data from the steers. Discriminant analysis was also conducted on the bull data for comparison. In the steers, 8 out of the 14 genes were significantly (P < 0.05) correlated with RFI-EBV. Two genes from the glutathione S-transferase mu family (GSTM1 and GSTM2) and the S100 calcium binding protein A10 (S100A10) had the highest correlations with RFI-EBV, with correlation coefficients of 0.59, 0.44 and 0.36, respectively. Based on the 14 expressed genes, 84% of the steers and 98% of the bulls were correctly classified into their respective RFI selection lines. The results of this study indicate that a high proportion of the genes that were differentially expressed in the original study with bulls were also differentially expressed in this study with steers. The high accuracy in classification obtained in this study shows that the transcriptional approach to the study of the biological processes involved in variation in RFI has great potential for identification of candidate genes.en
dc.languageenen
dc.publisherCSIRO Publishingen
dc.relation.ispartofAnimal Production Scienceen
dc.titleUsing genes differentially expressed in bulls to classify steers divergently selected for high and low residual feed intakeen
dc.typeJournal Articleen
dc.identifier.doi10.1071/AN11266en
dc.subject.keywordsAnimal Breedingen
local.contributor.firstnameYen
local.contributor.firstnamePaul Fen
local.contributor.firstnameRobert Men
local.contributor.firstnameKimen
local.contributor.firstnameI Men
local.subject.for2008070201 Animal Breedingen
local.subject.seo2008830301 Beef Cattleen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailpaul.arthur@industry.nsw.gov.auen
local.profile.emailrherd3@une.edu.auen
local.profile.emailkim.quinn@dpi.nsw.gov.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20130621-013014en
local.publisher.placeAustraliaen
local.format.startpage608en
local.format.endpage612en
local.peerreviewedYesen
local.identifier.volume52en
local.identifier.issue7en
local.contributor.lastnameChenen
local.contributor.lastnameArthuren
local.contributor.lastnameHerden
local.contributor.lastnameQuinnen
local.contributor.lastnameBarchiaen
dc.identifier.staffune-id:rherd3en
local.profile.orcid0000-0003-4689-5519en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:14473en
local.identifier.handlehttps://hdl.handle.net/1959.11/14258en
dc.identifier.academiclevelAcademicen
local.title.maintitleUsing genes differentially expressed in bulls to classify steers divergently selected for high and low residual feed intakeen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.description.statisticsepubsVisitors: 242<br />Views: 241<br />Downloads: 1en
local.search.authorChen, Yen
local.search.authorArthur, Paul Fen
local.search.authorHerd, Robert Men
local.search.authorQuinn, Kimen
local.search.authorBarchia, I Men
local.uneassociationUnknownen
local.year.published2012en
local.subject.for2020300305 Animal reproduction and breedingen
local.subject.seo2020100401 Beef cattleen
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