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|Title:||Using genes differentially expressed in bulls to classify steers divergently selected for high and low residual feed intake||Contributor(s):||Chen, Y (author); Arthur, Paul F (author); Herd, Robert M (author) ; Quinn, Kim (author); Barchia, I M (author)||Publication Date:||2012||DOI:||10.1071/AN11266||Handle Link:||https://hdl.handle.net/1959.11/14258||Fields of Research (FoR) 2008:||070201 Animal Breeding||Fields of Research (FoR) 2020:||300305 Animal reproduction and breeding||Socio-Economic Objective (SEO) 2008:||830301 Beef Cattle||Socio-Economic Objective (SEO) 2020:||100401 Beef cattle||Abstract:||Feed efficiency is an economically important trait in livestock and residual feed intake (RFI) is a commonly used measure of the trait in beef cattle. Residual feed intake is the difference between the actual feed intake recorded over a test period and the expected feed intake of an animal based on its size and growth rate. It is a heritable trait, and efficient animals have lower RFI values. Several genes have been identified as being differentially expressed in the liver of Angus bulls that have been divergently selected for RFI. The objective of this study was to use genes that are differentially expressed in bulls to classify Angus steers from the same divergent RFI selection lines. Liver samples were collected at slaughter from 40 high RFI and 40 low RFI steers that were ≈23 months old, and had just completed a 251-day feedlot feeding period. RNA samples from the livers were assayed by quantitative real-time PCR for 14 genes, which have been identified previously in bulls. Steers were not measured for RFI, hence the estimated breeding values (EBV) for RFI of their parents were used to calculate their mid-parent (average of the two parents) RFI-EBV. Correlation and discriminant analyses were conducted on the normalised quantitative real-time PCR data from the steers. Discriminant analysis was also conducted on the bull data for comparison. In the steers, 8 out of the 14 genes were significantly (P < 0.05) correlated with RFI-EBV. Two genes from the glutathione S-transferase mu family (GSTM1 and GSTM2) and the S100 calcium binding protein A10 (S100A10) had the highest correlations with RFI-EBV, with correlation coefficients of 0.59, 0.44 and 0.36, respectively. Based on the 14 expressed genes, 84% of the steers and 98% of the bulls were correctly classified into their respective RFI selection lines. The results of this study indicate that a high proportion of the genes that were differentially expressed in the original study with bulls were also differentially expressed in this study with steers. The high accuracy in classification obtained in this study shows that the transcriptional approach to the study of the biological processes involved in variation in RFI has great potential for identification of candidate genes.||Publication Type:||Journal Article||Source of Publication:||Animal Production Science, 52(7), p. 608-612||Publisher:||CSIRO Publishing||Place of Publication:||Australia||ISSN:||1836-5787
|Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal|
|Appears in Collections:||Journal Article|
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