Cost-Effective Microsatellite Markers for 'Banksia integrifolia' (Proteaceae)

Title
Cost-Effective Microsatellite Markers for 'Banksia integrifolia' (Proteaceae)
Publication Date
2013
Author(s)
Fatemi, Mohammad
( author )
OrcID: https://orcid.org/0000-0001-6618-4234
Email: mfatemi2@une.edu.au
UNE Id une-id:mfatemi2
Houliston, Gary J
Haddadchi, Azadeh
Gross, Caroline L
( author )
OrcID: https://orcid.org/0000-0001-8014-1548
Email: cgross@une.edu.au
UNE Id une-id:cgross
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Botanical Society of America
Place of publication
United States of America
DOI
10.3732/apps.1200130
UNE publication id
une:14191
Abstract
• Premise of the study: Microsatellite markers were developed to assess the level of genetic variation and population structure in 'Banksia integrifolia', a widespread species endemic to eastern Australia. • Methods and Results: We used next-generation sequencing approaches to identify and develop 11 polymorphic microsatellite markers with perfect tri- and tetranucleotide repeats. We tested these markers with 71 specimens from three populations. Observed and expected heterozygosities ranged from 0.0 to 0.875 and 0.0 to 0.763, respectively. • Conclusions: The developed markers will be valuable for studies of the population structure, mating system, and selection of provenances for restoration projects involving 'B. integrifolia'.
Link
Citation
Applications in Plant Sciences, 1(2), p. 1-3
ISSN
2168-0450
Start page
1
End page
3

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