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https://hdl.handle.net/1959.11/13153
Title: | A Flavoprotein Encoded in 'Selenomonas ruminantium' Is Characterized after Expression in 'Escherichia coli' | Contributor(s): | Anderson, Peter J (author); Cole, Lindsay J (author); McKay, David B (author); Entsch, Barrie (author) | Publication Date: | 2002 | DOI: | 10.1006/prep.2001.1581 | Handle Link: | https://hdl.handle.net/1959.11/13153 | Abstract: | 'Selenomonas ruminantium' is an obligate anaerobe that is very important for the provision of vitamin B12 to ruminants, which are particularly dependent upon this cofactor. One important use for vitamin B12 in anaerobic bacteria is for the utilization of glycerol as carbon source. A new flavoprotein has been found expressed by 'Escherichia coli' from a plasmid created as part of a gene library of 'S. ruminantium'. The 2.5-kb fragment of chromosomal DNA responsible for protein expression contains parts of two operons. Only one polypeptide (the flavoprotein) encoded by the 'S. ruminantium' DNA is produced in 'E. coli' in large amounts. The gene for the flavoprotein has been identified and is probably transcribed as part of an operon involved in glycerol metabolism in 'S. ruminantium'. The flavoprotein has been purified and its molecular properties have been examined. Sequence analysis showed that this protein is a divergent member of the family of nitroreductases. Pure protein is a homodimer with a molecular weight of 44,500, containing one molecule of FMN per dimer. Like other nitroreductases, this protein forms a complex with pyridine nucleotide (NADPH), but unlike other nitroreductases, it fails to be reduced in this complex at a biologically significant rate. It has none of the common catalytic properties of other members of the nitroreductase family. | Publication Type: | Journal Article | Source of Publication: | Protein Expression and Purification, 24(3), p. 429-438 | Publisher: | Academic Press | Place of Publication: | United States of America | ISSN: | 1096-0279 1046-5928 |
Fields of Research (FoR) 2008: | 060199 Biochemistry and Cell Biology not elsewhere classified | Socio-Economic Objective (SEO) 2008: | 970106 Expanding Knowledge in the Biological Sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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