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https://hdl.handle.net/1959.11/13011
Title: | Nitric Oxide and Nitroxides Can Act as Efficient Scavengers of Protein-Derived Free Radicals | Contributor(s): | Lam, Magdalena (author); Pattison, David (author); Bottle, Steven E (author); Keddie, Daniel (author); Davies, Michael (author) | Publication Date: | 2008 | DOI: | 10.1021/tx800183t | Handle Link: | https://hdl.handle.net/1959.11/13011 | Abstract: | Nitric oxide (•NO) may act as either a pro-oxidant or an antioxidant in biological systems. Although •NO and nitroxide radicals react slowly with most molecules, they react at near diffusion-controlled rates with other radicals and may therefore be efficient protective agents. This study assessed the ability of •NO and nitroxides to intercept specific protein-derived radicals and compared the efficacy of these species. Three protein radical systems were investigated as follows: BSA-derived radicals generated via radical transfer from H₂O₂-activated horseradish peroxidase, radicals formed on myoglobin via reaction with H₂O₂, and carbon-centered radicals formed from amino acid hydroperoxides on exposure to Fe²⁺-EDTA. In each case, radicals were generated in the absence or presence of •NO or nitroxides of different size and charge. Concentration-dependent loss of the protein radicals was detected by electron paramagnetic resonance with both •NO and nitroxides and time-dependent consumption of •NO using an •NO electrode. The protein oxidation product dityrosine was significantly reduced by •NO and nitroxides, and 3,4-dihydroxyphenylalanine levels were reduced by nitroxides but not •NO. Overall, these studies demonstrate that •NO and nitroxides are efficient near-stoichiometric scavengers of protein radicals and, hence, are potential protective agents against protein oxidation reactions and resulting damage. These reactions show little dependence on nitroxide structure or charge. | Publication Type: | Journal Article | Grant Details: | ARC/CE0561607 | Source of Publication: | Chemical Research in Toxicology, 21(11), p. 2111-2119 | Publisher: | American Chemical Society | Place of Publication: | United States of America | ISSN: | 1520-5010 0893-228X |
Fields of Research (FoR) 2008: | 060199 Biochemistry and Cell Biology not elsewhere classified 030501 Free Radical Chemistry 030503 Organic Chemical Synthesis |
Socio-Economic Objective (SEO) 2008: | 970103 Expanding Knowledge in the Chemical Sciences 970106 Expanding Knowledge in the Biological Sciences |
Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Science and Technology |
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