Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/127
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Renz, K | en |
dc.contributor.author | Islam, A | en |
dc.contributor.author | Cheetham, BF | en |
dc.contributor.author | Walkden-Brown, SW | en |
dc.date.accessioned | 2008-05-05T10:20:00Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Journal of Virological Methods, 135(2), p. 186-191 | en |
dc.identifier.issn | 1879-0984 | en |
dc.identifier.issn | 0166-0934 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/127 | - |
dc.description.abstract | Methods for Taqman quantitative real-time PCR (qPCR) assays to detect the three serotypes of Marek's disease virus (MDV) are available, and absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Using plasmid DNA, the lower detection limit of the MDV2 assay was determined to be 10 copies. Three independent assay runs showed highly reproducible Ct values and calculated copy numbers, with mean intra- and inter-assay coefficients of variation of less than 3% for Ct and less than 21.5% for calculated copy number. Absolute quantification of MDV2 was performed successfully on dust samples collected from poultry farms across Australia, material from infectious spleens and feather tips from chickens vaccinated with an attenuated strain of MDV2. Thus, it is now possible to use qPCR assays for absolute quantification of all three serotypes of MDV in a sample. | en |
dc.language | en | en |
dc.publisher | Elsevier BV | en |
dc.relation.ispartof | Journal of Virological Methods | en |
dc.title | Absolute quantification using real-time polymerase chain reaction of Marek's disease virus serotype 2 in field dust samples, feather tips and spleens | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1016/j.jviromet.2006.03.017 | en |
dc.subject.keywords | Virology | en |
local.contributor.firstname | K | en |
local.contributor.firstname | A | en |
local.contributor.firstname | BF | en |
local.contributor.firstname | SW | en |
local.subject.for2008 | 060506 Virology | en |
local.subject.seo | 630106 Poultry | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Science and Technology | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | krenz@une.edu.au | en |
local.profile.email | bcheetha@une.edu.au | en |
local.profile.email | swalkden@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | pes:3223 | en |
local.publisher.place | Netherlands | en |
local.format.startpage | 186 | en |
local.format.endpage | 191 | en |
local.identifier.scopusid | 33745133657 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 135 | en |
local.identifier.issue | 2 | en |
local.contributor.lastname | Renz | en |
local.contributor.lastname | Islam | en |
local.contributor.lastname | Cheetham | en |
local.contributor.lastname | Walkden-Brown | en |
dc.identifier.staff | une-id:krenz | en |
dc.identifier.staff | une-id:aislam2 | en |
dc.identifier.staff | une-id:bcheetha | en |
dc.identifier.staff | une-id:swalkden | en |
local.profile.orcid | 0000-0002-0638-5533 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:126 | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Absolute quantification using real-time polymerase chain reaction of Marek's disease virus serotype 2 in field dust samples, feather tips and spleens | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Renz, K | en |
local.search.author | Islam, A | en |
local.search.author | Cheetham, BF | en |
local.search.author | Walkden-Brown, SW | en |
local.uneassociation | Unknown | en |
local.identifier.wosid | 000238965800008 | en |
local.year.published | 2006 | en |
Appears in Collections: | Journal Article |
Files in This Item:
File | Description | Size | Format |
---|
SCOPUSTM
Citations
31
checked on Mar 23, 2024
Page view(s)
1,438
checked on Mar 17, 2024
Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.