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Title: Effect of passive immunization against leptin on ovarian follicular development in prepubertal mice
Contributor(s): Panwar, Shalini (author); Herrid, Muren  (author); Kauter, Kathleen (author); McFarlane, James R (author)orcid 
Publication Date: 2012
DOI: 10.1016/j.jri.2012.07.004
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Abstract: Leptin has been demonstrated to be essential for reproduction. However, all the relevant studies reported to date have investigated either the effect of a complete absence of leptin both centrally and peripherally, or excess leptin administration. The aim of this present study was to investigate the effect of reducing peripheral leptin concentrations on ovarian follicular development in prepubertal animals via administration of an anti-leptin antibody. Pre-pubertal female mice were administered anti-leptin antibody under the skin behind the head for four days, with or without gonadotropins, and ovaries were weighed and collected for follicle counting. Control animals were treated with non-immune serum. Passive immunization against leptin, with or without gonadotropins, resulted in a significant increase in ovarian weight compared with control ovaries. Furthermore, the ovaries from the anti-leptin group had significantly greater numbers of primary follicles per ovarian section than the control group, thus suggesting an increase in the transition of primordial to primary follicles after treatment. Interestingly, animals treated with anti-leptin plus gonadotropins had a significantly higher number of Graafian follicles in their ovaries compared with the other groups. Collectively, the results of the present study indicate that reduction of leptin in the circulation promotes ovarian follicle development in female mice, suggesting that peripheral leptin acts as an inhibitor of ovarian follicle development.
Publication Type: Journal Article
Source of Publication: Journal of Reproductive Immunology, 96(1-2), p. 19-24
Publisher: Elsevier Ireland Ltd
Place of Publication: Ireland
ISSN: 0165-0378
Field of Research (FOR): 111404 Reproduction
110306 Endocrinology
Socio-Economic Objective (SEO): 920106 Endocrine Organs and Diseases (excl. Diabetes)
920114 Reproductive System and Disorders
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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Appears in Collections:Journal Article
School of Environmental and Rural Science

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