Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/12376
Title: Fine-mapping the POLL locus in Brahman cattle yields the diagnostic marker CSAFG29
Contributor(s): Mariasegaram, Maxy (author); Harrison, Blair E (author); Bolton, Jennifer A (author); Tier, Bruce  (author); Henshall, John M (author); Barendse, William (author); Prayaga, Kishore C (author)
Publication Date: 2012
DOI: 10.1111/j.1365-2052.2012.02336.x
Handle Link: https://hdl.handle.net/1959.11/12376
Abstract: The POLL locus has been mapped to the centromeric region of bovine chromosome 1 (BTA1) in both taurine breeds and taurine–indicine crosses in an interval of approximately 1 Mb. It has not yet been mapped in pure-bred zebu cattle. Despite several efforts, neither causative mutations in candidate genes nor a singular diagnostic DNA marker has been identified. In this study, we genotyped a total of 68 Brahman cattle and 20 Hereford cattle informative for the POLL locus for 33 DNA micro satellites, 16 of which we identified de novo from the bovine genome sequence, mapping the POLL locus to the region of the genes IFNAR2 and SYNJ1. The 303-bp allele of the new micro satellite, CSAFG29, showed strong association with the POLL allele. We then genotyped 855 Brahman cattle for CSAFG29 and confirmed the association between the 303-bp allele and POLL. To determine whether the same association was found in taurine breeds, we genotyped 334 animals of the Angus, Hereford and Limousin breeds and 376 animals of the Brangus, Drought master and Santa Gertrudis composite taurine–zebu breeds. The association between the 303-bp allele and POLL was confirmed in these breeds; however, an additional allele (305 bp) was also associated but not fully predictive of POLL. Across the data, CSAFG29 was in sufficient linkage disequilibrium to the POLL allele in Australian Brahman cattle that it could potentially be used as a diagnostic marker in that breed, but this may not be the case in other breeds. Further, we provide confirmatory evidence that the scur phenotype generally occurs in animals that are heterozygous for the POLL allele.
Publication Type: Journal Article
Source of Publication: Animal Genetics, 43(6), p. 683-688
Publisher: Wiley-Blackwell Publishing Ltd
Place of Publication: United Kingdom
ISSN: 1365-2052
0268-9146
Fields of Research (FoR) 2008: 060412 Quantitative Genetics (incl Disease and Trait Mapping Genetics)
Fields of Research (FoR) 2020: 310506 Gene mapping
Socio-Economic Objective (SEO) 2008: 830301 Beef Cattle
Socio-Economic Objective (SEO) 2020: 100401 Beef cattle
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Animal Genetics and Breeding Unit (AGBU)
Journal Article

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