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This study aimed to determine the dose-dependent relationship between oral doses of fenbendazole (FBZ) and the plasma concentration of its metabolites, oxfendazole (OFZ) and fenbendazole-sulphone (SUL). Twenty five, two year-old, Merino wethers were equally allocated to treatment groups of different oral dose rates of FBZ (n = 5) and housed in individual pens. Treatment groups were designed to provide daily oral doses of 0.25, 0.5, 1.0, 2.0 and 4.0 mg/kg live weight of FBZ, suspended in water, for six days. Blood samples were collected from each animal at 48, 96, and 144 hours after administration of FBZ. Plasma was equally combined within each animal and analysed to determine concentrations of FBZ, OFZ and SUL. There was a positive linear relationship between FBZ dose rate and FBZ metabolite plasma concentration (R² = 0.991, P <0.001). Mean separation of plasma concentrations indicated significant differences (P <0.05) between treatments designed to provide 1.0, 2.0 or 4.0 mg/kg/day FBZ. Plasma concentrations of animals which received 0.25 or 0.50 mg/kg/day FBZ were significantly lower than other treatments (P <0.05). The results from this experiment provide preliminary support for the investigation of FBZ as a useful marker to estimate supplement intake of grazing animals. |
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