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https://hdl.handle.net/1959.11/10777
Title: | Transgenic Overexpression of Tartrate-Resistant Acid Phosphatase Is Associated with Induction of Osteoblast Gene Expression and Increased Cortical Bone Mineral Content and Density | Contributor(s): | Gradin, P (author); Hollberg, K (author); Cassady, Alan (author); Lang, P (author); Andersson, G (author) | Publication Date: | 2012 | DOI: | 10.1159/000330806 | Handle Link: | https://hdl.handle.net/1959.11/10777 | Abstract: | Bone remodeling is a central event in the maintenance of skeletal tissue, and involves cycles of resorption followed by the formation of bone tissue. The activity of osteoclasts and osteoblasts during these cycles is tightly regulated by systemic and local factors coupling the action of these cells. Tartrate-resistant acid phosphatase (TRAP) is predominantly expressed in bone by osteoclasts but has also been detected in osteoblasts and osteocytes. Moreover, TRAP can stimulate the differentiation of mesenchymal lineage cells, i.e. progenitors of osteoblasts and adipocytes. In order to further explore the effects of TRAP on bone turnover, the structural and molecular phenotypes of osteoclasts and osteoblasts were assessed in TRAP-overexpressing transgenic mice. Transgenic mice of both sexes display increased cortical bone mineral content and density, which cannot be accounted for by decreased bone resorption since osteoclast numbers and resorptive activity do not differ from wild-type mice. Examination of the osteoblast phenotype revealed that markers of bone formation, i.e. procollagen type I N-terminal propeptides, and osteoblast lineage markers as well as the TRAP 1B mRNA transcript are increased in TRAP-overexpressing mice. Expression of the osteoclast-selective TRAP 1C mRNA is not increased in TRAP transgenic mice. Elevated expression of TRAP mRNA and protein were detected in osteoblasts, osteocytes and in the bone matrix of TRAP transgenic mice, suggesting that TRAP overexpression in osteoblast lineage cells is associated with increased cortical bone mineral content and density. The data presented here support the hypothesis that TRAP overexpression in the osteoblastic cell lineage stimulates the differentiation and/or activation of these cells. | Publication Type: | Journal Article | Source of Publication: | Cells, Tissues, Organs, 196(1), p. 143-156 | Publisher: | S Karger AG | Place of Publication: | Switzerland | ISSN: | 1422-6421 1422-6405 |
Fields of Research (FoR) 2008: | 060103 Cell Development, Proliferation and Death | Fields of Research (FoR) 2020: | 310102 Cell development, proliferation and death | Socio-Economic Objective (SEO) 2008: | 920108 Immune System and Allergy | Socio-Economic Objective (SEO) 2020: | 200101 Diagnosis of human diseases and conditions | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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