Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/10626
Title: A study into the hypoglycaemic effectiveness of a combination of traditional Chinese herbs (GLUCOSTAT) in mouse heart tissue culture
Contributor(s): Collins, Mechele J (author); McFarlane, James R (author)orcid 
Publication Date: 2008
Handle Link: https://hdl.handle.net/1959.11/10626
Abstract: We have previously demonstrated that a combination of traditional Chinese herbs and chromium nicotinamide available commercially Australia (GLUCOSTAT) was effective in lowering fasted blood glucose concentrations in patients showing early signs of type 2 diabetes (1). However this treatments mode of action remains unknown. The aim of this study was to investigated its effectiveness in stimulating glucose uptake into muscle tissue using mouse heart muscle as the experimental model. An aqueous extract was prepared by placing 5l of the herb combination into 1 litre boiling water and allowing it to stand for 24 hours. The extract was filtered and kept at -20C until Male Swiss mice were euthanased and the heart removed and placed into ice cold saline. The heart was cut into 2mm squares and place 1 ml DMEM per well in 24 well tissue culture plates. Various concentrations of the herbal extract was added to each well in quadrupli in the presence of two concentrations of glucose (4 & 8 mM/l). The plates were incubated for 24 hours in 5% CO₂ in air at 37C. glucose concentration was then measured by a Dade XL clinical analyser and the results expressed as a percentage of glucose relative to heart tissue without the presence of the herbal extract. The results showed that the herbal extract had a negligible effect (2% 4mM/l of glucose but significantly increased glucose uptake at 8mM/l (15%). These results suggest that this combination of traditional Chinese herbs may be effective in stimulating glucose uptake by muscle tissue in moderately elevated glucose concentrations. Further is required to investigate the mechanism.
Publication Type: Conference Publication
Conference Name: Australian Diabetes Society (ADS) and Australian Diabetes Educators Association (ADEA) Annual Scientific Meeting, Melbourne, Australia, 27th - 29th August, 2008
Conference Details: Australian Diabetes Society (ADS) and Australian Diabetes Educators Association (ADEA) Annual Scientific Meeting, Melbourne, Australia, 27th - 29th August, 2008
Source of Publication: Australian Diabetes Society (ADS) and Australian Diabetes Educators Association (ADEA) Annual Scientific Meeting Proceedings and Abstract Book, p. 128-128
Publisher: Australian Diabetes Society (ADS) and Australian Diabetes Educators Association (ADEA)
Place of Publication: Australia
Field of Research (FOR): 060603 Animal Physiology - Systems
Socio-Economic Outcome Codes: 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Category Description: E3 Extract of Scholarly Conference Publication
Statistics to Oct 2018: Visitors: 164
Views: 162
Downloads: 0
Appears in Collections:Conference Publication

Files in This Item:
2 files
File Description SizeFormat 
Show full item record

Page view(s)

36
checked on Mar 3, 2019
Google Media

Google ScholarTM

Check


Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.