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Title: Pathogenicity and Infection Cycle of 'Vibrio owensii' in Larviculture of the Ornate Spiny Lobster ('Panulirus ornatus')
Contributor(s): Goulden, Evan F (author); Hall, M R (author); Bourne, D G (author); Pereg, Lily  (author); Hoj, L (author)
Publication Date: 2012
Open Access: Yes
DOI: 10.1128/AEM.07274-11Open Access Link
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Abstract: The type strain of 'Vibrio owensii' (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster ('Panulirus ornatus'). 'V. owensii' DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism 'Artemia' (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled 'V. owensii' DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. 'V. owensii' DY05 is a marine enteropathogen highly virulent to 'P. ornatus' phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species.
Publication Type: Journal Article
Source of Publication: Applied and Environmental Microbiology, 78(8), p. 2841-2849
Publisher: American Society for Microbiology
Place of Publication: United States of America
ISSN: 1098-5336
Field of Research (FOR): 070401 Aquaculture
060504 Microbial Ecology
060501 Bacteriology
Socio-Economic Outcome Codes: 830106 Aquaculture Rock Lobster
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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