Non-destructive quantification of cereal roots in soil using high-resolution X-ray tomography

Title
Non-destructive quantification of cereal roots in soil using high-resolution X-ray tomography
Publication Date
2012
Author(s)
Flavel, Richard
( author )
OrcID: https://orcid.org/0000-0001-7867-2104
Email: rflavel3@une.edu.au
UNE Id une-id:rflavel3
Guppy, Christopher
( author )
OrcID: https://orcid.org/0000-0001-7274-607X
Email: cguppy@une.edu.au
UNE Id une-id:cguppy
Tighe, Matthew
Watt, Michelle
McNeill, Ann
Young, Iain
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Oxford University Press
Place of publication
United Kingdom
DOI
10.1093/jxb/err421
UNE publication id
une:10465
Abstract
One key constraint to further understanding plant root development is the inability to observe root growth in situ due to the opaque nature of soil. Of the present non-destructive techniques, computed tomography (CT) is best able to capture the complexities of the edaphic environment. This study compared the accuracy and impact of X-ray CT measurement of in situ root systems with standard technology (soil core washing and WinRhizo analysis) in the context of treatments that differed in the vertical placement of phosphorus fertilizers within the soil profile. Although root lengths quantified using WinRhizo were 8% higher than that observed in the same plants using CT, measurements of root length by the two methodologies were highly correlated. Comparison of scanned and unscanned plants revealed no effect of repeated scanning on plant growth and CT was not able to detect any changes in roots between phosphorus treatments that was observed using WinRhizo. Overall, the CT technique was found to be fast, safe, and able to detect roots at high spatial resolutions. The potential drawbacks of CT relate to the software to digitally segment roots from soil and air, which will improve significantly as automated segmentation algorithms are developed. The combination of very fast scans and automated segmentation will allow CT methodology to realize its potential as a high-throughput technique for the quantification of roots in soils.
Link
Citation
Journal of Experimental Botany, 63(7), p. 2503-2511
ISSN
1460-2431
0022-0957
Start page
2503
End page
2511

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