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Title: Non-destructive quantification of cereal roots in soil using high-resolution X-ray tomography
Contributor(s): Flavel, Richard  (author); Guppy, Christopher  (author)orcid ; Tighe, Matthew  (author); Watt, Michelle  (author); McNeill, Ann (author); Young, Iain  (author)
Publication Date: 2012
Open Access: Yes
DOI: 10.1093/jxb/err421Open Access Link
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Abstract: One key constraint to further understanding plant root development is the inability to observe root growth in situ due to the opaque nature of soil. Of the present non-destructive techniques, computed tomography (CT) is best able to capture the complexities of the edaphic environment. This study compared the accuracy and impact of X-ray CT measurement of in situ root systems with standard technology (soil core washing and WinRhizo analysis) in the context of treatments that differed in the vertical placement of phosphorus fertilizers within the soil profile. Although root lengths quantified using WinRhizo were 8% higher than that observed in the same plants using CT, measurements of root length by the two methodologies were highly correlated. Comparison of scanned and unscanned plants revealed no effect of repeated scanning on plant growth and CT was not able to detect any changes in roots between phosphorus treatments that was observed using WinRhizo. Overall, the CT technique was found to be fast, safe, and able to detect roots at high spatial resolutions. The potential drawbacks of CT relate to the software to digitally segment roots from soil and air, which will improve significantly as automated segmentation algorithms are developed. The combination of very fast scans and automated segmentation will allow CT methodology to realize its potential as a high-throughput technique for the quantification of roots in soils.
Publication Type: Journal Article
Source of Publication: Journal of Experimental Botany, 63(7), p. 2503-2511
Publisher: Oxford University Press
Place of Publication: United Kingdom
ISSN: 0022-0957
Field of Research (FOR): 050399 Soil Sciences not elsewhere classified
070303 Crop and Pasture Biochemistry and Physiology
Socio-Economic Outcome Codes: 961499 Soils not elsewhere classified
820507 Wheat
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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