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Title: Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice
Contributor(s): Wallach, Michael G (author); Webby, Richard J (author); Islam, A Fakhrul  (author); Walkden-Brown, Steve W  (author)orcid ; Emmoth, Eva (author); Feinstein, Ricardo (author); Gronvik, Kjell-Olov (author)
Publication Date: 2011
Open Access: Yes
DOI: 10.1128/CVI.05075-11Open Access Link
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Abstract: Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of in vitro assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an in vivo mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both in vitro and in vivo. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection.
Publication Type: Journal Article
Source of Publication: Clinical and Vaccine Immunology, 18(7), p. 1083-1090
Publisher: American Society for Microbiology
Place of Publication: Washington DC, United States
ISSN: 1556-679X
Field of Research (FOR): 070205 Animal Protection (Pests and Pathogens)
070705 Veterinary Immunology
070712 Veterinary Virology
Socio-Economic Objective (SEO): 830309 Poultry
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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Appears in Collections:Journal Article
School of Environmental and Rural Science

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