Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice

Title
Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice
Publication Date
2011
Author(s)
Wallach, Michael G
Webby, Richard J
Islam, A Fakhrul
Walkden-Brown, Steve W
( author )
OrcID: https://orcid.org/0000-0002-0638-5533
Email: swalkden@une.edu.au
UNE Id une-id:swalkden
Emmoth, Eva
Feinstein, Ricardo
Gronvik, Kjell-Olov
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
American Society for Microbiology
Place of publication
United States of America
DOI
10.1128/CVI.05075-11
UNE publication id
une:10323
Abstract
Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of in vitro assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an in vivo mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both in vitro and in vivo. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection.
Link
Citation
Clinical and Vaccine Immunology, 18(7), p. 1083-1090
ISSN
1556-679X
1556-6811
Start page
1083
End page
1090

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