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Title: Fluorescence correlation spectroscopy, Raster image correlation spectroscopy and Number & Brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1)
Contributor(s): Moens, Pierre  (author)orcid ; Gratton, Enrico (author); Salvemini, Iyrri (author)
Publication Date: 2010
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Abstract: Fluorescence correlation spectroscopy (FCS) was developed in 1972 by Magde, Elson and Webb (Magde et al., 1972). Photon counting detectors and avalanche photodiodes have become standards in FCS to the point that there is a widespread belief that these detectors are essential to perform FCS experiments, despite the fact that FCS was developed using analog detectors. Spatial and temporal intensity fluctuation correlations using analog detection on a commercial Olympus Fluoview 300 microscope has been reported by Brown et al. (2008). However, each analog instrument has its own idiosyncrasies that need to be understood before using the instrument for FCS. In this work we explore the capabilities of the Nikon C1, a low cost confocal microscope, to obtain single point FCS, Raster-scan Image Correlation Spectroscopy and Number & Brightness data both in solution and incorporated into the membrane of Giant Unilamellar Vesicles (GUVs).
Publication Type: Conference Publication
Conference Name: Biomedical Imaging Symposium - "From seeing to believing: Quantitative Microscopy", Sydney, Australia, 24th September, 2010
Conference Details: Biomedical Imaging Symposium - "From seeing to believing: Quantitative Microscopy", Sydney, Australia, 24th September, 2010
Grant Details: NHMRC/568301
Source of Publication: Biomedical Imaging Symposium Program, p. 2-2
Publisher: University of New South Wales
Place of Publication: Online
Field of Research (FOR): 029901 Biological Physics
Socio-Economic Outcome Codes: 970110 Expanding Knowledge in Technology
HERDC Category Description: E3 Extract of Scholarly Conference Publication
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