Interactions Between Profilin And Polyphosphatidylinositol Lipids

Title
Interactions Between Profilin And Polyphosphatidylinositol Lipids
Publication Date
2008
Author(s)
Moens, Pierre
( author )
OrcID: https://orcid.org/0000-0003-3121-5306
Email: pmoens@une.edu.au
UNE Id une-id:pmoens
Krishnan, Kannan
Bagatolli, Luis A
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
Cell Press
Place of publication
United States of America
Series
Biophysical Society Annual Meeting Abstracts
DOI
10.1016/S0006-3495(08)79122-7
UNE publication id
une:10237
Abstract
Profilin is a small (12-15 kDa) actin binding protein. Profilin is also involved in the signaling pathway linking receptors in the cell membrane to the microfilament system within the cell. Profilin is thought to play critical roles in this signaling pathway through its interaction with polyphosphoinositides (PPI). To date, profilin's interaction with PPI has only been studied in micelles or small vesicles. Profilin binds with high affinity to small clusters of phosphatidylinositol (4, 5) bis-phosphate [PI(4, 5)P₂] molecules. In this work, we investigated the interactions of profilin with sub-micellar concentrations of PI(4, 5)P₂ and PI(3, 4, 5)P₃. Fluorescence anisotropy was used to determine the relevant dissociation constants for binding of sub-micellar concentrations of fluorescently labeled PPI lipids to profilin and we show that these are significantly different from those determined for profilin interaction with micelles or small vesicles. Despite the low affinity for submicellar concentration of PI(4, 5)P₂, profilin was shown to bind to Giant unilamellar vesicles in presence of 1% mole fraction of PI(4, 5)P₂ Forster Resonance Energy Transfer experiments indicate that profilin is able to recruit adjacent PI(4, 5)P₂ molecules after initial interaction with the GUV membrane. The implications of these findings are discussed.
Link
Citation
Biophysical Journal, 94(2, Supplement), p. 691-691
ISSN
1542-0086
0006-3495
Start page
691
End page
691

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