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Title: Interactions Between Profilin And Polyphosphatidylinositol Lipids
Contributor(s): Moens, Pierre (author)orcid ; Krishnan, Kannan (author); Bagatolli, Luis A (author)
Publication Date: 2008
DOI: 10.1016/S0006-3495(08)79122-7
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Abstract: Profilin is a small (12-15 kDa) actin binding protein. Profilin is also involved in the signaling pathway linking receptors in the cell membrane to the microfilament system within the cell. Profilin is thought to play critical roles in this signaling pathway through its interaction with polyphosphoinositides (PPI). To date, profilin's interaction with PPI has only been studied in micelles or small vesicles. Profilin binds with high affinity to small clusters of phosphatidylinositol (4, 5) bis-phosphate [PI(4, 5)P₂] molecules. In this work, we investigated the interactions of profilin with sub-micellar concentrations of PI(4, 5)P₂ and PI(3, 4, 5)P₃. Fluorescence anisotropy was used to determine the relevant dissociation constants for binding of sub-micellar concentrations of fluorescently labeled PPI lipids to profilin and we show that these are significantly different from those determined for profilin interaction with micelles or small vesicles. Despite the low affinity for submicellar concentration of PI(4, 5)P₂, profilin was shown to bind to Giant unilamellar vesicles in presence of 1% mole fraction of PI(4, 5)P₂ Forster Resonance Energy Transfer experiments indicate that profilin is able to recruit adjacent PI(4, 5)P₂ molecules after initial interaction with the GUV membrane. The implications of these findings are discussed.
Publication Type: Conference Publication
Conference Name: 52nd Annual Meeting of the Biophysical Society and 16th International Biophysics Congress, Long Beach, United States of America, 2nd - 6th February, 2008
Source of Publication: Biophysical Journal, 94(2, Supplement), p. 691-691
Publisher: Cell Press
Place of Publication: United States of America
ISSN: 0006-3495
Field of Research (FOR): 060101 Analytical Biochemistry
029901 Biological Physics
HERDC Category Description: E3 Extract of Scholarly Conference Publication
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Series Name: Biophysical Society Annual Meeting Abstracts
Series Number : 2008
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