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Title: Interactions of human profilin-1 and phosphatidylinositol 4,5-bisphosphate in giant unilamellar vesicles
Contributor(s): Krishnan, Kannan (author); Cody, Stephen H (author); Clayton, Andrew H A (author); Moens, Pierre (author)orcid 
Publication Date: 2007
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Abstract: Human Profilin-I is a small actin binding proteins which also interact with polyphosphoinositides (PPI) (Lassing & Lindberg, 1985) and proline rich motif containing proteins. Profilin is involved in the signaling pathway linking the receptors in the cell membrane to the microfilament system. Profilin is thought to play critical roles in this signaling pathway through its interaction with phosphatidylinositol 4,5-bisphosphate [PI(4,5)P₂] (Lu et al., 1996). So far, profilin's interaction with PPI has only been studied in micelles or small vesicles. Profilin binds with high affinity to small clusters of PI(4,5)P₂ molecules (Goldschmidt-Clermont et al., 1991). In cells, the organization of PPI lipids is different from micelles, therefore the interaction with profilin might be quite different. We have used giant unilamellar vesicles (GUV) as a membrane model system to investigate the interactions between PI(4,5)P₂ and profilin. BODIPY® TMR PI(4,5)P₂C16 (Rhodamine labelled 16-carbon fatty acid containing Phosphatidylinositol 4, 5-bisphosphate) was incorporated into GUV membrane. Confocal images of GUVs were obtained in presence and absence of profilin. These images were analysed using SimFCS software (Prof. Enrico Gratton, Laboratory for Fluorescence Dynamics, Irvine, USA). The diffusion coefficient and the aggregation of PIP2 in the membrane were determined and profilin had a clear effect upon the diffusion coefficient and aggregation of PIP2.
Publication Type: Conference Publication
Conference Name: Joint Annual Meeting of the Australian Physiological Society (AuPS) and Australian Society for Biophysics (ASB), Newcastle, Australia, 2nd - 5th December, 2007
Source of Publication: Proceedings of the Australian Physiological Society, v.38, p. 8-8
Publisher: Australian Physiological Society
Place of Publication: Australia
Field of Research (FOR): 060101 Analytical Biochemistry
029901 Biological Physics
HERDC Category Description: E3 Extract of Scholarly Conference Publication
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Series Name: Proceedings of the Australian Physiological Society
Series Number : 38
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