Chen, Letian

Hamada, Satoshi

Kawasaki, Tsutomu

Shimamoto, Ko

Fujiwara, Masayuki

Zhu, Tingheng

Thao, Nguyen Phuong

Wong, Hann Ling

Krishna, Priti

Ueda, Takashi

Kaku, Hanae

Shibuya, Naoto

2010

Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs) represents a critical first step of innate defense in plants and animals. However, maturation and transport of PRRs are not well understood. We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER). Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sar1, a small GTPase which regulates ER-to-Golgi trafficking. Further, Hop/Sti1 and Hsp90 are present at the PM in a complex (designated the "defensome") with OsRac1, a plant-specific Rho-type GTPase. Finally, Hop/Sti1 was required for chitin-triggered immunity and resistance to rice blast fungus. Our results suggest that the Hop/Sti1-Hsp90 chaperone complex plays an important and likely conserved role in the maturation and transport of PRRs and may function to link PRRs and Rac/Rop GTPases.

Cell Host & Microbe, 7(3), p. 185-196

1934-6069

1931-3128

https://hdl.handle.net/1959.11/15567

en

Cell Press

The Hop/Sti1-Hsp90 Chaperone Complex Facilitates the Maturation and Transport of a PAMP Receptor in Rice Innate Immunity

Journal Article

Publication