Comparison of tracheal and choanal cleft swabs and poultry dust samples for detection of Newcastle disease virus and infectious bronchitis virus genome in vaccinated meat chicken flocks

Title
Comparison of tracheal and choanal cleft swabs and poultry dust samples for detection of Newcastle disease virus and infectious bronchitis virus genome in vaccinated meat chicken flocks
Publication Date
2021-04-16
Author(s)
Assen, Awol M
Walkden-Brown, Stephen
( author )
OrcID: https://orcid.org/0000-0002-0638-5533
Email: swalkden@une.edu.au
UNE Id une-id:swalkden
Stillman, Mark
Alfirevich, Sheridan
Gerber, Priscilla F
( author )
OrcID: https://orcid.org/0000-0002-8343-8299
Email: pgerber2@une.edu.au
UNE Id une-id:pgerber2
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Public Library of Science
Place of publication
United States of America
DOI
10.1371/journal.pone.0247729
UNE publication id
une:1959.11/40361
Abstract
This study assessed different methods (tracheal and choanal cleft swabs from individual birds, and poultry dust as a population level measure) to evaluate the shedding kinetics of infectious bronchitis virus (IBV) and Newcastle disease virus (NDV) genome in meat chicken flocks after spray vaccination at hatchery. Dust samples and tracheal and choanal cleft swabs were collected from four meat chicken flocks at 10, 14, 21 and 31 days post vaccination (dpv) and tested for IBV and NDV genome copies (GC) by reverse transcriptase (RT)-PCR. IBV and NDV GC were detected in all sample types throughout the study period. Detection rates for choanal cleft and tracheal swabs were comparable, with moderate and fair agreement between sample types for IBV (McNemar's = 0.27, kappa = 0.44) and NDV (McNemar's = 0.09; kappa = 0.31) GC respectively. There was no significant association for IBV GC in swabs and dust samples (R2 = 0.15, P = 0.13) but NDV detection rates and viral load in swabs were strongly associated with NDV GC in dust samples (R2 = 0.86 and R2 = 0.90, P<0.001). There was no difference in IBV and NDV GC in dust samples collected from different locations within a poultry house. In conclusion, dust samples collected from any location within poultry house show promise for monitoring IBV and NDV GC in meat chickens at a population level and choanal cleft swabs can be used for detection of IBV and NDV GC instead of tracheal swabs in individual birds.
Link
Citation
PLoS One, 16(4), p. 1-11
ISSN
1932-6203
Start page
1
End page
11
Rights
Attribution 4.0 International

Files:

NameSizeformatDescriptionLink
openpublished/ComparisonAssenWalkdenBrownFreitasGerber2021JournalArticle.pdf 592.141 KB application/pdf Published version View document