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This dataset accompanies a PhD thesis on photosystem II (PSII) heat tolerance in wheat and barley at the University of New England, Australia. PSII heat tolerance was measured as the critical temperature (Tcrit) marking the onset of the rise in basal chlorophyll fluorescence. Field data include Tcrit, PSII oxygen evolution, dark respiration and grain yield in wheat and barley. Wheat experimental data include phenotypic measurements, RNA-sequencing gene counts, normalised gene expression, homoeologue expression states and triad-to-gene mappings. The spectral dataset contains 3,421 paired measurements of leaf hyperspectral reflectance and Tcrit. Additional Tcrit observations include experimental identifiers and quality-control annotations. These data support analyses of genetic variation in PSII heat tolerance, relationships with physiological traits and yield, gene-expression responses to heatwave exposure, and prediction of Tcrit from leaf reflectance. The data are organised by thesis chapter, with a README, worksheet index, data dictionary, and RNA sample key. |
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