| Title |
|
Understanding and improving direct UV detection of monosaccharides and disaccharides in free solution capillary electrophoresis |
|
|
| Publication Date |
|
| Author(s) |
|
| Type of document |
|
| Language |
|
| Entity Type |
|
| Publisher |
|
| Place of publication |
|
| DOI |
|
10.1016/j.aca.2013.12.001 |
|
|
| UNE publication id |
|
| Abstract |
|
Direct UV detection of carbohydrates in free solution capillary electrophoresis at 270 nm is made possible by a photo-oxidation reaction. Glucose, rhamnose and xylose were shown to have unique UV absorption spectra hypothesizing different UV absorbing intermediates for their respective photo-oxidation. NMR spectroscopy of the photo-oxidation end products proved they consisted of carboxylates and not malondialdehyde as previously theorized and that oxygen thus plays a key role in the photo-oxidation pathway. Adding the photo-initiator Irgacure® 2959 in the background electrolyte increased sensitivity by 40% at an optimum concentration of 1 x 10⁻⁴mM and 1 x 10⁻⁸mM for conventional 50 μm i.d. capillaries and for the corresponding extended light path capillaries, respectively. |
|
|
| Link |
|
| Citation |
|
Analytica Chimica Acta, v.809, p. 183-193 |
|
|
| ISSN |
|
| Start page |
|
| End page |
|