| Author(s) |
Oliver, James D
Rosser, Adam A
Fellows, Chris
Guillaneuf, Yohann
Clement, Jean-Louis
Gaborieau, Marion
Castignolles, Patrice
|
| Publication Date |
2014
|
| Abstract |
Direct UV detection of carbohydrates in free solution capillary electrophoresis at 270 nm is made possible by a photo-oxidation reaction. Glucose, rhamnose and xylose were shown to have unique UV absorption spectra hypothesizing different UV absorbing intermediates for their respective photo-oxidation. NMR spectroscopy of the photo-oxidation end products proved they consisted of carboxylates and not malondialdehyde as previously theorized and that oxygen thus plays a key role in the photo-oxidation pathway. Adding the photo-initiator Irgacure® 2959 in the background electrolyte increased sensitivity by 40% at an optimum concentration of 1 x 10⁻⁴mM and 1 x 10⁻⁸mM for conventional 50 μm i.d. capillaries and for the corresponding extended light path capillaries, respectively.
|
| Citation |
Analytica Chimica Acta, v.809, p. 183-193
|
| ISSN |
1873-4324
0003-2670
|
| Link | |
| Language |
en
|
| Publisher |
Elsevier BV
|
| Title |
Understanding and improving direct UV detection of monosaccharides and disaccharides in free solution capillary electrophoresis
|
| Type of document |
Journal Article
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| Entity Type |
Publication
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