Prevalence of Campylobacter and non-typhoidal Salmonella along broiler chicken production and distribution networks, Northern Vietnam

2025-11-05

Xin, Chen

Hoang,Thuy Thi

Truong, Duong Quy

Tran, Nhat Thi

Pham, Hoa Thi Thanh

Dang, Son Thi Thanh

Luu, Huong Quynh

Hong Le, Nhung Thi

Butt, Saira

Tran, Hang Thi Thu

Seow, Kelyn

Hennessey, Mathew

Lehri, Burhan

Lam, Chun Ting Angus

Conway, Patricia Lynne

Stabler, Richard A

Blake, Damer

Tomley, Fiona

Pfeiffer, Dirk

Pham, Ngoc Thi

Fournié, Guillaume

Conan, Anne

Journal Article

en

Publication

Public Library of Science

United States of AMerica

10.1371/journal.pntd.0013615

une:1959.11/71672

Campylobacter and non-typhoidal Salmonella (NTS) are among the most common foodborne pathogens found in chickens at any production stage and cause gastroenteritis in humans. This study aimed to estimate the prevalence of Campylobacter spp. (C. coli and C. jejuni) and NTS in broiler production and distribution networks (PDNs) using a Bayesian approach. It also investigated the NTS serotypes circulating in these PDNs. A cross-sectional study was conducted in four provinces in northern Vietnam between March 2021 and March 2022. A total of 102 sites, including live bird markets, slaughter facilities (slaughterhouses and slaughter points), and their supplying farms, were randomly selected for sampling. Cecal and environmental samples were cultured for isolation of Campylobacter and NTS, with serotypes of NTS determined by targeted analysis of whole genome sequences. Bayesian models were developed to estimate the prevalence of Campylobacter at two levels (bird-level and site-level) and NTS at site-level. The selected best-fitted models indicated that C. jejuni prevalence was primarily influenced by site type, while C. coli was affected by both province and site types. For NTS, only site type was included. The highest overall prevalence of infected broilers was estimated on farms for C. coli (26.2% [95% High Density Interval (HDI): 19.0-36.0%]) and C. jejuni (19.9% [95%HDI 13.0-27.0%]). Slaughter points (97.6% [95%HDI 63.3-99.9%]) and wholesale markets (91.7% [95%HDI 28.2-99.9%]) had the highest probability of C. coli and C. jejuni contamination, respectively, but retail markets had the highest proportion of infected broilers at contaminated sites. NTS contamination was more frequent in markets and slaughter facilities (42.8% [95%HDI 30.8-57.1%]) than on farms (18.6% [95%HDI 9.5-30.1%]). Among 16 detected NTS serotypes, S. Infantis and S. Kentucky were the most common. These findings highlight the widespread contamination of broiler PDNs with Campylobacter and NTS in northern Vietnam, emphasizing the need for enhanced surveillance and control measures in PDNs to mitigate the risk of foodborne transmission.

link

PLoS Neglected Tropical Diseases, 19(11), p. 1-19

1935-2735

1935-2727

1

19

Attribution 4.0 International